期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

大肠杆菌细菌生物膜菌落计数实验技术改良初探 被引量：5

下载PDF
导出
摘要 目的:应用高速离心技术对大肠杆菌生物被膜(biofilm,BF)菌落计数方法进行改良,为大肠杆菌BF实验提供更稳定、更简便计数条件。方法:采用1.5%腹膜透析液(乳酸盐)-腹膜透析留置管系统培养大肠杆菌BF,建模3d。以对载体的处理时间不同设立对照组(30min)和改良组(13min),分别采用现有实验技术方法及使用高速离心技术改良方法处理BF内活菌,计算BF内活菌落数。结果:经两种方法处理后,计数菌落数差异无统计学意义(P>0.05),且改良组[(5.96±0.320)lgCFU/mL]较对照组[(5.92±0.470)lgCFU/mL]实验数据结果离散程度更小,改良组较对照组实验时间更为节约。结论:生物被膜菌落计数实验,改良实验技术更优于现有技术。
作者 邹龙涛 伍巧源 李艳林 彭茜 廖蕴华
机构地区 广西医科大学第一附属医院肾内科
出处 《广西医科大学学报》 CAS 2012年第4期639-640,共2页 Journal of Guangxi Medical University
基金 2010年广西教育厅科研项目(201010LX038) 2010年广西壮族自治区中医药管理局科研项目(GZZC1132)
关键词 大肠杆菌 生物被膜 技术改良
分类号 R466 [医药卫生—临床医学]
  • 相关文献

参考文献8

  • 1Favre-Bonte S.Kohler T, Van Delden C. Biofilm formation by Pseudomonas aeruginosa: role of the C4-HSL cell-to-cell signal and inhibition by azithromycin [ J]. Antimicrob Chemother,2003,52(4) : 598-604.
  • 2Donlan RM. Hiofilms : microbial life on surfaces[J]. Em-erg Infect Dis,2002,8(9) :881-890.
  • 3lshida H,Ishida Y, Kurosaka Y,et al. In vitro and in vivo activities of levofloxacin against biofilm-producing Pseudomonas aeruginosa[J]. Antimicrob Agents Che-mother. 1998,42(7) : 1 641-1645.
  • 4孔晋亮,刘晓岚,陈一强,王立赞,闫萍,覃雪军,朱莲娜.黄芩水煎液联合左氧氟沙星对铜绿假单胞菌生物被膜的影响[J].天津医药,2008,36(5):331-333. 被引量：21
  • 5王成玉,梁俐,伍巧源,薛超,邹龙涛,廖蕴华.在腹膜透析液及LB液环境中腹膜透析留置管大肠杆菌生物被膜形成能力的对比[J].内科,2011,6(5):397-399. 被引量：8
  • 6Stewart PS.Costerton JW. Antibiotic resistance of bacteria in hiofilms[J]. Lancet,2001 ,358(9276) . 135-138.
  • 7Johnson LL. Peterson RV, Pitt WG. Treatment of bacterial hiofilms on polymeric biomaterials using antibiotics and ultrasound[J]. Biomater Sci Polym Ed. 1998 , 9 (11):1 177-1 185.
  • 8Sauer K , Camper AK , Ehrlich GI), et al. Pseudomonas aeruginosa displays multiple phenotypes during development as a biofilm[J]. Bacteriol,2002,184(4): 1 140-1 154.

二级参考文献12

  • 1孔晋亮,刘晓岚,陈一强,王立赞,闫萍,覃雪军,朱莲娜.黄芩水煎液对生物被膜的抑制作用及对左氧氟沙星的增效作用[J].中华微生物学和免疫学杂志,2007,27(3):241-241. 被引量：1
  • 2Donlan RM. Biltilms: microbial life on surfaces [ J ]. Emerg Infect Dis,2002,8(9) :881 -890.
  • 3Dasqupta MK. Strategies for intervention in and prevention of biofilmrelated infection in peritoneal dialysis patients[ J]. Adv Petit Dial, 2003,19 : 195 - 197.
  • 4Passaricllo C,Berlutti F,Selan L,et al. A rapid staining procedure to demonstrate glycocalyx production and bacterial biofilms [ J ]. New Microbiol, 1994,17 (3) :225 - 230.
  • 5Dong YH, Wang LH, Xu JL, et al. Quenching quorum-sensingdependent bacterial infection by an N-acyl homoserine lactonase [ J ]. Nature ,2001,411 (6839) :813 - 817.
  • 6Dasgupta MK. Biofflms and infection in dialysis patients [ J ]. Semin Dial,2002,15 (5) :338 -346.
  • 7Sepandj F, Ceri H, Gibb A, et al. Minimum inhibitory concentration (MIC) versus minimum biofilm eliminating concentration ( MBEC ) in evaluation of antibiotic sensitivity of gram-negative bacilli causing peritonitis[ J]. Petit Dial Int,2004,24( 1 ) :65 - 67.
  • 8Dasgupta MK, Larabie M. Biofilms in peritoneal dialysis [ J ]. Petit Dial hat ,2001,21 ( Suppl 3 ) :213 - 217.
  • 9Favre-Bonte S, Kohler T, Van Delden C. Biofilm formation by Pseudomonas aeruginosa:role of the C4-HSL cell-to-cell signal and inhibition by azithromycin[J]. Antimicrobial Chemother, 2003,52(4) : 598-604.
  • 10Nagata T, Mukae H, Kadota J,et al. Effect of Erythromycin on Chronic Respiratory Infection Caused by Pseudomonas aeruginosa with Biofilm Formation in an Experimental Murine Model[J]. Antimicrob Agents Chemother, 2004,48 (6) : 2251-2259.

共引文献27

同被引文献33

  • 1陈海荣,刘美娟,林松娟,郑世良,刘玉波.盐酸氨溴索联合阿米卡星对铜绿假单胞菌成熟生物膜的体外影响[J].潍坊医学院学报,2009,31(2):152-154. 被引量：4
  • 2叶晓光,王若伦,林红燕,魏衍超.铜绿假单胞菌耐药性动态变化特征及分析[J].中华医院感染学杂志,2004,14(6):607-609. 被引量：66
  • 3Donln R M. Biofilms: microbial life on surfaces [J]. Emerg Infect Dis, 2002, 8(9): 881-890.
  • 4Piao Z, Sze C C, Barysheva O, et al. Temperature-regulated formation of mycelial mat-like biofilms by legionellapneumophila [J]. Appl Environ Microbiol, 2006, 72(2): 1613-1622.
  • 5Skyberg J A, Siek K E, Doetkott C, et al. Biofilm formation by avian Escherichia coli in relation to media, source and phylogeny[J]. J Appl Microbial, 2007, 102(2): 548-554.
  • 6Srdjan S, Dragana V. A modified microtiter-plate test for quantification of Staphylococcal biofilm formation [J]. J Microbiol Meth, 2000, 40(2):175-179.
  • 7Favre-Bonte S, Kohler T, Van Delden C. Biofilm forma- tion by Pseudomonas aeruginosa: role of the C4-HSL cell-to-cell signal and inhibition by azithromycin[J]. J Antimicrob Chemother,2003,52(4) :598-604.
  • 8Dasgupta MK. Strategies for intervention in and preven tion of biofilm-related infection in peritoneal dialysis pa- tients[J]. Adv Perit Dial,2003,19 : 195-197.
  • 9Ishida H,Ishida Y,Kurosaka Y,et al. In vitro and in vi vo activities of levofloxaein against biofilm-producing Pseudomonas aeruginosa[J]. Antimicrob Agents Che- mother, 1998,42(7) : 1641-1645.
  • 10Jacobsen SM,Stickler DJ, Mobley HI., et al. Complicat ed catheter-associated urinary tract infections due to Escherichia coli and Proteus mirabilis[J]. Clin Microbi ol Rev,2008,21(l) :26-59.

引证文献5

二级引证文献27

广西医科大学学报
2012年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部