血浆梅毒螺旋体DNA含量与潜伏梅毒患者传染性的相关分析

Correlated Research on Treponema Pallidum DNA Level in Plasma and Infectivity of Latent Syphilis Patients

目的观察潜伏梅毒患者血浆中梅毒螺旋体DNA(TPDNA)含量与传染性的相关性。方法应用荧光定量PCR法检测32例潜伏梅毒患者及其固定性伴侣血浆中TPDNA含量。结果 TPDNA阳性13例,DNA为5.26×102～2.56×104copies/mL;其中16例的性伴侣确诊为梅毒,其TPDNA阳性6例,DNA为1.23×102～3.34×104copies/mL。早期梅毒患者性伴侣的感染率(61.1%)高于晚期梅毒患者(35.7%)。TPDNA含量高的患者性伴的梅毒感染率较高,相应的TPDNA检出率亦较高。女性TPDNA的阳性率高于男性(P<0.05)。结论潜伏梅毒患者的传染性与血浆中TPDNA含量有关。

Objective To observe the correlation between Treponema pallidum （TP） DNA level in plasma and infectivity of latent syphilis patients. Methods Plasma level of TP DNA were detected by fluorescent quantitation polymerase chain reaction （FQ-PCR） in 32 latent syphilis patients and their stabile sex partners. Results Positive rate of TP DNA was 40.6% of 32 latent syphilis patients, with TP DNA were 5.26 x 102^2 - 2.56 ×10^4 copies/mL. Sixteen partners were diagnosed syphilis, positive rate of TP DNA was 18.75% , with TP DNA were 1.23 ×10^2-3.34 × 10^4 copies/mL. The infective rate of early patients was higher than late ones （61.1%/35.7%）. Patients with higher TP DNA level, the infective rate and detection rate of partners also higher. Positive rate of TP DNA of female was higher than male （P 〈 0.05 ）. Conclusion The infectiviy of latent syphilis patients is related to plasma TP DNA level.