栉孔扇贝消化系统γ-氨基丁酸的免疫组织化学定位

Immunohistochemical Localization of Gamma-aminobutyric Acid in the Digestive System of Chlamys farreri

采用抗生物素-生物素-过氧化物酶法(ABC法)的免疫组织化学技术,对γ-氨基丁酸(GABA)在栉孔扇贝(Chlamys farreri)消化系统各组织器官中的分布进行了定位研究。结果表明,栉孔扇贝的唇瓣、口唇、肠道的上皮细胞均呈GABA阳性反应,阳性反应物质呈颗粒状分布且多集中于上皮细胞游离端;胃上皮中未见GABA阳性反应;肝胰腺的上皮细胞及部分结缔组织中呈现GABA阳性反应,其中部分上皮细胞中的阳性反应物质呈团块状分布。GABA在栉孔扇贝消化系统除胃以外的各器官均有分布,推测其可能参与消化功能的调节作用。

The gamma-aminobutyric acid （GABA） in the digestive system of Chlamys farreri was analyzed by immunohistochemical ABC staining. The results showed that GABA-positive cells existed widely in the epithelium of labellum, labia oris, and intestine. Besides, the positive granules were centralized in the dissociative sides of epithelium. There were no GABA-positive cells in the epithelium of stomach. Positive cells were also observed in both the epithelium and connective tissue of hepatopancreas, and some of them were accumulated in lumps in the epithelium. It can be concluded that GABA is abundantly distributed in the digestive system in C.farreri and it may be involved in the regulation of digestive function.