低氧对甘肃鼢鼠体内ACTH及血液生理指标的影响

Effect of Hypoxia on ACTH and Blood Parameters in Gansu Zokor

为研究低氧对甘肃鼢鼠(Myospalax cansus)血清促肾上腺皮质激素(ACTH)及血液生理指标的影响,采用KX-21N全自动血液分析仪对甘肃鼢鼠低氧处理前后血液中红细胞数(RBC)﹑血红蛋白浓度(HGB)﹑红细胞压积(HCT)、平均红细胞体积(MCV)、平均血红蛋白含量(MCH)及平均血红蛋白浓度(MCHC)进行测定,并采用SN-695A型智能放免r测量仪测定血清中ACTH浓度。结果表明,氧浓度分别为14.1%、10.5%和6.5%条件下,甘肃鼢鼠RBC、HGB和HCT均显著升高,MCV变化不明显,MCH和MCHC下降,血清ACTH含量显著升高;腹腔注射ACTH后甘肃鼢鼠RBC、HGB、HCT均显著升高。说明低氧激活了甘肃鼢鼠下丘脑-垂体-肾上腺皮质轴(HPA),导致ACTH分泌增加,刺激皮质酮分泌,致RBC、HGB、HCT含量增加,从而提高血液中红细胞的携氧能力以适应低氧生活环境。

To study the effects of hypoxia on adrenocorticotropic hormone （ACTH）and blood parameters in Gansu Zokor （Myospalax cansus） , we measured the number of red blood cell （RBC） , hemoglobin concentration （HGB） , hematocrit （ I-ICT ） , mean corpuscular volume （ MCV ） , mean corpuscular hemoglobin （ MCH ） , and mean corpuscular hemoglobin concentration （MCHC）contents by using Sysmex KX-21N hematology analyzer before and after the hypoxia treatment,and also measured the adrenocorticotropic hormone（ACTH） contents by SN-695A RIA -y measuring instrument before and after hypoxia. The value of RBC, HGB, HCT,and ACTH were significantly higher in the condition that oxygen concentration was 14. 1%, 10.5%, and 6.5%, while the measurement of MCV did not change significantly, MCH and MCHC decreased after hypoxia adaptation. After intraperitoneal injection of ACTH,the RBC, HGB, and HCT contents all increased evidently. Hypoxia activated hypothalamus-pituitary-adrenal（HPA） and increased ACTH contents in serum,further increased corticosterone （CORT）. Those change resulted in the higher value of RBC, HGB, and HCT that would improve the oxygen carrying capacity of blood and adaptation ability of Gansu Zokor to hypoxia.