肝癌细胞疫苗对荷瘤小鼠脾淋巴细胞IFN-γ生成及肿瘤浸润淋巴细胞的影响

Influence of tumor cell vaccine on lymphocyte IFN-γ production and tumor infiltrating lymphocytes in tumor-bearing mice

目的探讨高强度聚焦超声(HIFU)制备肿瘤疫苗对小鼠抗同源肿瘤免疫力的影响。方法以HIFU辐照或高温水浴灭活H22细胞制成HIFU瘤苗和高温瘤苗。将雌性BALB/c小鼠108只,随机分为HIFU瘤苗、高温瘤苗、生理盐水组,分别皮下注射HIFU瘤苗、高温瘤苗、生理盐水,之后接种H22细胞,观测荷瘤小鼠肿瘤体积及生存期。ELSIA检测免疫后荷瘤小鼠脾淋巴细胞在同源肿瘤再刺激下IFN-γ的生成。免疫组化检测小鼠肿瘤浸润T淋巴细胞。结果 HIFU瘤苗免疫使小鼠肿瘤生长被抑制,脾淋巴细胞IFN-γ生成增加,且较高温瘤苗组更显著(P<0.05)。瘤苗增加了肿瘤中CD4+和CD8+细胞浸润的程度。结论 HIFU瘤苗提高了实验动物的抗肿瘤免疫力,表现为增高的淋巴细胞IFN-γ生成和肿瘤内淋巴细胞浸润。

Objective To investigate the influence of tumor vaccine prepared by high intensity focused ultrasound（HIFU） on an ti-homologous tumor immunity in mice. Methods HIFU tumor vaccine and hypertherrnic tumor vaccine were prepared by inactiva ting H22 cells with the HIFU irradiation or hyperthermic water bath. 108 female BALB/c mice were randomly divided into HIFU tumor vaccine,hyperthermic tumor vaccine and normal saline groups,20 mice in each group. Then H22 murine hepatic carcinoma cells were inoculated subcutaneously. The tumor volume and lifetime of all tumor-bearing mice were observed. The IFN-7 production by mice spleen lymphocytes under the re-stimulation of H22 cell was measured by ELISA, and tumor infiltrating lymphocytes （TIL） were investigated by immunohistochemistry. Results The tumor growth of mice was suppressed by HIFU tumor vaccine immunity and the IFN-7 production of spleen lymphocytes was increased, which were more significant than hyperthermic tumor vaccine（P〈0.05）. More CD4＋ and CD8＋ cells were found in tumors of vaccines immunized mice. Conclusion HIFU prepared tumor vaccine enhances the mice anti-tumor immunity manifested by increasing IFN-7 production of lymphocytes and TILs.