Pre-miR-10a重组腺病毒载体对K562细胞增殖的抑制作用

Inhibitory effect of the recombinant adenovirus pre-miR-10a on K562 cell proliferation

目的:观察在K562细胞高表达miR-10a对细胞增殖及凋亡的影响。方法:用pAd-pre-miR-10a重组腺病毒载体感染K562细胞,采用RT-PCR检测感染后K562细胞中miR-10a和bcr/abl融合基因的表达水平,Western blot法检测BCR/ABL融合蛋白表达水平,MTT、流式细胞术(FCM)分别检测感染后K562细胞的增殖及凋亡。结果:与对照组相比,K562细胞在转染pAd-pre-miR-10a重组腺病毒载体后,其miR-10a表达水平明显升高、bcr/abl融合基因水平显著降低;BCR/ABL融合蛋白表达明显下调、K562细胞增殖活力被抑制、细胞凋亡增加,差异均具有统计学意义(P<0.05)。结论:pAd-pre-miR-10a重组腺病毒载体能明显抑制K562细胞的增殖、促进细胞凋亡。

AIM： To investigate the changes of prolifer- ation and apoptosis in K562 cells over-expressing miR-10a. METHODS： K562 cells were infected with pAd-pre-miR-10a virus, and then the levels of miR-10a and bcr/abl in trans- fected K562 cells was detected using RT-PCR and the level of BCR/ABL using Western blotting. The methylthiazolyl tetrazolium （Ml-r） assay and flow cytometry （FCM） were used to monitor the changes of proliferation and apoptosis of K562 cells after transfection. RESULTS： Compared with the control groups, K562 cells transfected with pAd-pre-miR-10a presented the significantly increased level of miR-10a, the significantly decreased expressions of bcr/abl fusion gene and BCR./ABL fusion protein, the inhibited proliferation abili- ty and the promoted apoptosis （ P 〈 0.05）. CONCLUSION： The recombinant adenovirus of pAd-pre-miR-10a can inhibit the proliferation of K562 cells and promote cell apoptosis significantly.