摘要
利用茶树[Camellia sinensis(L.)O.Kuntze]品种"农抗早"的叶片进行培养,诱导形成愈伤组织。选择生长旺盛、致密的愈伤组织块与根癌农杆菌EHA105进行共培养,通过潮霉素(Hygromycin)抗性筛选、GUS染色及PCR的验证,获得部分转基因愈伤组织。在添加100μmol·L-1的乙酰丁香酮(AS)处理后,转化率约为10%。同时试验结果证明,AS可以提高农杆菌的转化效率,增加渗透压却不能提高转化率。
Calli were induced from leaves of tea cultivar ＇Nongkangzao＇, and those growing vigorously with compact tissue were chosen for agrobacterium-mediated transformation by EHA105. The transgenic callus was screened using hygromycin. The resistant callus was confirmed by RT-PCR and GUS dyeing. The results showed that transgenic callus was obtained using this method, and the conversion rate was about 10% after adding 100 μmol.L-1acetosyringone （AS）. The results also showed that the efficiency of agrobacterium-mediated transforma- tion could be effectively improved by adding AS, but the increase in osmotic pressure had no effect.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2012年第5期721-724,共4页
Journal of Anhui Agricultural University
基金
国家自然科学基金项目(31070614)
安徽农业大学人才引进基金共同资助
关键词
茶树
转基因
组织培养
愈伤组织
根癌农杆菌
Camellia sinensis
transgenosis
cell tissue culture
calli
Agrobacterium
