Toll样受体2与高迁移率族蛋白1在系统性红斑狼疮患者中的表达

The expression of TLR2 and high mobility group protein 1 in systemic lupus erythematosus

目的通过检测系统性红斑狼疮（SLE）患者血清中高迁移率族蛋白1（HMGB1）及其外周血单核细胞上Tou样受体2（TLR2）的表达，评价其在SLE病情进展中的作用。方法随机选取SLE患者40例，活动期与稳定期各20例，健康对照组20名，用酶联免疫吸附试验（ELISA）检测血清中HMGB1蛋白的表达，用流式细胞术分析外周血单核细胞上TLR2的表达，同时检测外周血补体C4、抗双链DNA（dsDNA）抗体、抗clq抗体、抗核小体抗体，采用单因素方差分析和KmskM。WMlis检验进行统计分析。结果3组血清HMGB1分别为：活动组HMGB-1[（48．9±11．3）μg/L]，稳定组[（14．8±1．9）μg/L]，对照组[（13．5±3．6）μg/L]，其中活动组血清HMGB-1表达水平较稳定组和对照组明显增高（P〈0．05）；外周血单核细胞TLR2表达率活动组[（96．7±1．3）％]，稳定组[（83．5±9．1）％]，对照组[（83．3±9．9）％]，其中活动组SLE患者TLR2的表达较稳定组SLE和对照组相比明显增高（P〈0．05）；血清中HMGB1的表达水平与单核细胞表面TLR2的表达呈正相关（r=0．551，P〈0．05）。结论血清HMGB1和外周血单核细胞表TLR2均参与了SLE的病情发展过程。

Objective To evaluate the pathogenesis and disease progression by detecting the expres- sion of Serum High mobility group protein 1 （HMGB1） and TLR2 in monocytes of patients with systemic lupus erythematosus （SLE）. Methods Forty patients with SLE were selected randomly , 20 patients were in active disease group and others were in stable disease group. The expression of HMGB1 in the serum of these cases were detected by enzyme linked immunosorbent assay （ELISA） and TLR2 on CD14± monocytes in the periphe- ral blood were detected by FCM. The correlation between these indexes and clinical, laboratory indexes about SLE were analyzed using one-way ANOVA, and Kruskal-Wallis test. Results The expression levels of HMGB-1 in serum was [（48.9±11.3） μg/L] in the active group, while that was [（14.8±1.9） μg/L] in the stable group was, and [（13.5±3.6） μg/L] in the control group. HMGB1 in the active SLE group was significantly higher （P〈0.05） when compared with that of the stable and control group. The expression of TLR2 in the peripheral blood mononuclear cells was [ （96.7±1.3）%.] in the active group, [ （83.5±9.1）% ] in the stable group, and [ （83.3±9.9）% ] in the control group TLR2 in the active SLE group was up-regulated when compared with the stable and control groups （P〉0.05）. There were positive correlation between the serum levels of HMGB1 and TLR2 in the peripheral blood mononuclear cells （r=0.551, P〈0.05）. Conclusion The expression levels of HMGB-1 in serum and the expression of TLR2 in peripheral blood mononuclear cells may participate in the pathological processes of SLE.