摘要
目的探讨他克莫司对淋巴细胞分泌IL-6和sIL-2R及表达1L-6和sIL-2RmRNA的影响。方法酶联免疫吸附试验测定不同浓度他克莫司对人Jurkat淋巴瘤细胞产生IL-6和sIL-2R的影响，实时荧光定量PCR法分析他克莫司对淋巴瘤细胞IL-6mRNA和sIL-2RmRNA表达的影响。结果102-10nmol／L他克莫司可以抑制Jurkat淋巴瘤细胞分泌IL-6、sIL-2R（P值均〈0．05），其中10^3-10^4 nmol／L他克莫司作用显著。10^2nmol／L他克莫司可下调淋巴瘤细胞IL-6和sIL-2RmRNA的表达（P值均〈0．05）。结论适当浓度他克莫司可抑制淋巴细胞分泌IL-6和sIL-2R，下调IL-6和sIL-2RmRNA的表达。
Objective To evaluate the effects of tacrolimus on the secretion of IL-6 and sIL-2R as well as the expression of IL-6 and sIL-2R mRNA by lymphocytes. Methods Jurkat human lymphoma cells were cultured and treated with tacrolimus of different concentrations. Enzyme linked immunosorbent assay was performed to determine the levels of IL-6 and sIL-2R in the supernatant of Jurkat cells at 48 hours after treatment with tacrolimus of 0, 10, 102, 103 and 104 nmol/L, and real time reverse transcription PCR to measure the expression of IL-6 mRNA and sIL-2R mRNA of Jurkat cells at 48 hours after treatment with tacrolimus of 102 nmol/L. Results Tacrolimus of 102 - l04 nmol/L could suppress the secretion of [L-6 and sIL-2R from Jurkat cells （all P 〈 0.05）, with a more marked suppressing effect achieved by the use of tacrolimus at 103 - 104 nmol/L. The expressions of IL-6 and sIL-2R mRNA from Jurkat cells were downregulated by tacrolimus of 102 nmol/L （both P 〈 0.05）. Conclusion Tacrolimus at certain concentrations could downregulate the secretion of IL-6 and slL-2R as well as the expression of IL-6 and slL-2R mRNA by lymphocytes
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2012年第10期745-746,共2页
Chinese Journal of Dermatology
基金
中华医学会皮肤性病学分会一安斯泰来皮肤病学研究基金
