唾液测试板检测口腔幽门螺杆菌的实用性研究

Study of the pragmatic of saliva test cassette to detect the Helicobacter pylori in oral cavity

目的探讨幽门螺杆菌唾液测试板(HPS)与碳-13尿素呼气试验(13C-UBT)检测口腔幽门螺杆菌的相关性及其诊断的特异性及灵敏性。方法选择13C-UBT检测阳性者106例和13C-UBT检测阴性者24例,共130例研究对象,采用HPS法进行口腔幽门螺杆菌检测。以13C-UBT为金标准,对HPS法检测结果用贝叶斯定理方法进行评价。结果HPS的灵敏度为68.87%,特异度为58.33%,准确度为66.92%,阳性预测值为87.95%,阴性预测值为29.79%。二者的符合率为66.92%。结论HPS法与13C-UBT法的检测结果具有一定的相关性,13C-UBT法对口腔幽门螺杆菌检测存在盲区,不适用于作为评价口腔幽门螺杆菌检测方法HPS的金标准。HPS法与13C-UBT法可临床上同步使用。

Objective To study the link of Helicobacter pylori salive test cassette（HPS） and ^3C-urea breath test （13C-UBT） on detecting Helicobacter pylori infection of stomach, and the sensitivity as well as specificity should be determined. Methods A screening trial of 13C-UBT and HPS tests was carried out 130 patients included 106 positive and 24 negative results of 13C-UBT. As gold standards of 13C-UBT, a comparison study with HPS was evaluated using Bayes formula. Results The sensitivity, specificity, accuracy, positive and negative predictive value of HPS was 68.87%, 58.33%, 66.92%, 87.95%, 29.79% respectively. The coincidence was 66.92%. Conclusion There is a correlation between results of 13C-UBT and HPS. Combination of 13C-UBT and HPS may compensate the blind zone of 13C-UBT in detecting oral Helicobacter pylori infection. 13C-UBT could not be used as a gold standard to judge the validation of HPS in detecting Helicobacter pylori oral infection. The methods can be used at the same time in clinic.