绿茶提取物表没食子儿茶素没食子酸酯对酸蚀症牙本质胶原降解作用的初步研究

Pilot study of the effect of green tea extractive epigallocatechin-3-gallate on degradation of collagen in dental erosion

目的观察绿茶提取物表没食子儿茶素没食子酸酯(EGCG)对酸蚀症暴露的牙本质胶原降解作用的影响。方法选取酸蚀症志愿者64名,随机分成两组。制作个别托盘,托盘内注入羧甲基纤维素钠(CMC)凝胶(对照组)或添加了EGCG的CMC凝胶(试验组)。要求志愿者于夜间睡时佩戴,清晨取下。试验前1d,以及试验后第1、2、3、4周收集培养液,用放射免疫试剂盒法测量Ⅰ型胶原羧基端肽(ICTP)的质量浓度。结果凝胶种类和使用时间对ICTP的质量浓度均有影响;EGCG能快速、有效地降低ICTP的表达水平,与对照组相比,差异有统计学意义(P<0.05)。结论EGCG能有效地减少脱矿牙本质胶原的降解,可以提高牙本质抗酸蚀的能力。

Objective To observe the effect of green tea extractive epigallocatechin-3-gallate （EGCG） on degradation of collagen in dental erosion. Methods Sixty-four volunteers with dental erosion were randomly divided into two groups. The custom fitted trays were made from the heat curable braces and were injected with carboxymethyl cellulose sodium（CMC） hydrogel in one group（control group） or CMC hydrogel with EGCG added in another（experi- mental group）. These trays were used during sleep and removed from oral cavity in the next morning. The content of carboxy-terminal telopeptide of type I collagen （ICTP） were determined by radioimmunoassay kit at 1 d before the experiment, and 1, 2, 3, 4 weeks after the experiment. Results Both the types and time showed significant effects on ICTP. The incubation in EGCG resulted in relatively rapid and significant （P〈0.05） decrease in the expression level of ICTP compared with the control group. Conclusion EGCG can inhibit collagen degradation and improve the anti- erosive effect of dentine.