冬凌草甲素及联合柔红霉素对jurkat细胞增殖抑制作用的观察

Growth inhibitory effects of oridonin alone or combined with DNR on jurkat cells

目的:探讨冬凌草甲素单用及联合柔红霉素对急性T淋巴细胞白血病jurkat细胞增殖抑制的影响。方法:采用CCK-8法检测单用冬凌草甲素、柔红霉素对jurkat细胞的抑制作用,及两种药物联合应用对jurkat细胞的抑制作用;采用Giemsa法检测冬凌草甲素作用于jurkat细胞48h的凋亡形态学变化;采用流式细胞仪检测单用冬凌草甲素对jur-kat细胞的凋亡率及两药物联合时的凋亡率。结果:单用不同浓度的冬凌草甲素(1、2、4、8和16μg/mL)对jurkat细胞具有增殖抑制作用,且呈时间-浓度依赖性,其中干预48h后的抑制率分别为(10.80±1.58)%、(32.32±7.83)%、(42.27±4.43)%、(55.07±1.65)%和(70.36±4.11)%;当联合小剂量柔红霉素(0.04μg/mL)时对jurkat细胞的抑制作用显著增加。Giemsa染色后,随着药物浓度的增加,镜下可观察到胞体皱缩、染色质浓集和凋亡小体等形态学变化。不同浓度的冬凌草甲素干预jurkat细胞48h后的凋亡率分别为(7.74±0.96)%、(13.26±1.49)%、(17.42±1.24)%、(25.13±2.12)%和(29.07±0.59)%,呈浓度依赖性增加;单用柔红霉素(0.04μg/mL)干预jurkat细胞48h后的凋亡率为(18.19±1.33)%;当冬凌草甲素(4μg/mL)联合柔红霉素(0.04μg/mL)干预jurkat细胞时,其凋亡率为(51.06±2.25)%,与单用两种药物相比差异有统计学意义,P<0.05。结论:冬凌草甲素能够抑制jurkat细胞增殖和诱导凋亡,联合柔红霉素对jurkat细胞的增殖抑制作用显著增强,具有协同抑制效应。

OBJECTIVE： To investigate the proliferation and apoptotic effects of oridonin alone or combined with DNR on jurkat cells. METHODS：CCK-8 was used to detect the proliferation inhibitory effects of oridonin alone or combined with DNR on jurkat cells and to observe the apoptotic morphologic changes of jurkat cells treated with different concentrations of oridonin for 48 h. FCM was used to exam the apoptotic rate of jurkat cells. RESULTS： The proliferation inhibitory effects of different concentrations of oridonin on jurkat cells were in a dose and time dependent manner and the inhibitory rates of oridonin treated for 48 hour were （10.80±1.58）%,（32. 32±7.83）%,（42.27±4.43）%, （55.07± 1.65）%, （70. 36± 4. 11）% respectively. The inhibitory effects markedly enhanced when combined with DNR （0.04 μg/mL）;the morphologic changes such as cell body shrinkage, neuclear condensation, apoptotic bodies were observed by Giemsa stain with the condensation increasing ; the apoptotic rate of different concentrations of oridonin on jurkat cells for 48 h were （7.74±0.96） %,（13.26±1.49）%,（17.42±1.24）%,（25.13±2.12）%, （29.07±0.59）% respectively increased in a dose-dependent manner. When jurkat cells were incubated with oridonin（4 μg/mL） and DNR （0.04 μg/mL） in combination, the apoptotic rate was （51. 06 ± 2. 25）%, which was higher than that of oridonin （4 μg/mL）and DNR（0.04 μg/mL） each alone and there was a significant difference （P〈0.05）. CONCLUSION： Orido- nin can inhibit jurkat cells proliferation and induce apoptosis in a does-dependent manner and the inhibitory effects markedly enhanced when combined with DNR,which has collaborative inhibition effect.