应用介入技术建立猪肝硬化门脉高压模型的实验研究

The establishment of liver cirrhosis along with portal hypertension model in experimental pigs by using interventional technique

目的构建肝硬化动物模型并分析其病理学改变,探讨介入技术建立肝硬化模型的可行性与科学性。方法将30头健康雄性贵州香猪分为实验组A 10头和实验组B 10头,分别采用四氯化碳腹腔注射法和肝动脉乙醇注入法构建肝硬化门脉高压症动物模型,另外10头作为对照组。结果实验组A和实验组B成模平均时间分别为12周和6周,成模率分别为80%和100%。动物模型的门脉压力,对照组为(8.2±2.3)cm水柱,实验组A为(12.4±2.2)cm水柱,实验组B为(12.6±2.1)cm水柱,实验组与对照组比较差异有统计学意义(P<0.05)。结论采用介入技术向肝动脉内灌注乙醇优于腹腔注射四氯化碳造模法,具有造模创伤小,全身损害轻、可重复性强、操作简单、病理表现及分期明显的优点,可以建立较为理想的肝硬化动物模型。

Objective To establish the liver cirrhosis along with portal hypertension model in experimental pigs by using interventional technique, to analyze its pathological changes, and to discuss the feasibility and rationality of this technique. Methods A total of 30 healthy Guizhou male pigs were divided into three groups： study group A（n = 10）, study group B（n = 10） and control group（n = 10）. Pigs in study group A received intraperitoneal injection of CCL4 and pigs in study group B received hepatic artery injection of alcohol in order to establish the models of liver cirrhosis complicated by portal hypertension. Results The average time for the establishment of model was 12 weeks in group A and 6 weeks in group B, and the model success rate was 80% andl00% respectively. The portal pressure of animal models in the control group was （8.2 ± 2.3） cm H20, which was （12.4 ± 2.2） cm H20 in study group A and was （12.6 ± 2.1） cm H2O in study group B. Statistically significant difference in portal pressure existed between the control group and both study groups （P 〈 0.05）. Conclusion For the establishment of liver cirrhosis along with portal hypertension model, hepatic arterial injection of alcohol is superior to intraperitoneal injection of CCL4. The technique of hepatic arterial injection of alcohol is minimally-invasive, easily manipulated and highly repeatable with less damage to the body. Besides, the models demonstrate clear pathologic features and staging.（J Intervent Radial, 2012, 21： 756-759）