温胆片对高架十字迷宫实验小鼠的抗焦虑作用

Anti-anxiety Effect of Wendan Tablets on Mice in Elevated Plus Maze Test

目的观察温胆片在小鼠高架十字迷宫实验中的抗焦虑作用。方法 50只小鼠随机分为正常对照组,地西泮组,温胆片低、中、高剂量组。其中温胆片低、中、高组分别按0.9,1.8,3.6 mg.kg-1剂量给小鼠灌胃温胆片混悬液,地西泮组予等容积地西泮混悬液灌胃,正常对照组灌服等容积生理盐水。连续给药10 d,采用小鼠高架十字迷宫装置,对小鼠进行行为测试。结果与正常对照组比较,地西泮组和温胆片各组的开放臂时间(OT)和开臂次数(OE)的百分率均有增高趋势,其中地西泮组和温高组的差异有统计学意义(均为P<0.05)。与正常对照组比较,地西泮组及温胆片各组的开放臂和封闭臂总次数(OE+CE)和向下探究次数(HD)值无统计学意义(P>0.05);与正常对照组比较,地西泮组和温胆片各组的封闭臂内后腿直立次数(RE)值均有下降的趋势,其中地西泮组及温胆片中、高剂量组显著高于正常对照组(P<0.05,P<0.01)。结论温胆片可增加高架十字迷宫小鼠的OT和OE值,但不增加OE+CE值,提示其有一定的抗焦虑作用;其作用强度与地西泮相似,呈量效关系,以温胆片高剂量组为优。

Objective To observe the anti-anxiety effect of Wendan tablets（WT） on mice in elevated plus maze test. Methods Fifty healthy male Kunming mice at SPF level were randomly divided into 5 groups： blank control group, Diazepam group （treated with suspension of Diazepam 1.5 mg. kg-1 orally） , and low-, moderate- and high-dose WT groups（treated with suspension of WT in the dosages of 0.9, 1.8 g and 3.6 mg. kg-1 orally respectively）. Blank control group was fed with equal volume of saline. The administration lasted 10 days, and behavioral tests were performed by using the elevated plus maze device for each group. Results Compared with blank control group, the percentages of open-area time（OT %） and close-area time（OE %） showed an increasing trend in Diazepam group and WT groups, and the difference was significant between western medicine group and high-dose WT group （P 〈 0.05 ）. Compared with Blank group, OE＋CE value and HD in the blank control group did not differ from those in western medicine group and WT groups （P 〉 0.05 ） . Compared with the blank control group, RE value in Diazepam group and WT groups showed a downward trend, and the difference among Diazepam group, moderate-dose and high-dose WT groups was statistically significant（P 〈 0.05 or P 〈 0.01 ）. Conclusion Wendan tablets shows certain anti-anxiety ef- fect by increasing OE % and OT % value, but has no effect on OE ＋ CE value. The effect is similar to that of diazepam, and is in dose-dependent manner.