摘要
目的通过体内和体外模型研究知母皂苷对芒果苷肠吸收的影响。方法采用液-质联用法测定大鼠灌胃芒果苷、知母皂苷-芒果苷后的血药浓度,并计算主要药动学参数;应用大鼠肠外翻模型,UPLC法测定不同时间点时肠囊内芒果苷的浓度,计算肠吸收参数。结果合用知母皂苷后,芒果苷的血药浓度比单体组明显升高,药时曲线下面积(AUC)和Cmax显著增加,Tmax延长至3 h;在回肠段知母皂苷-芒果苷(1∶1)组中芒果苷的吸收参数Q90、Vt和Paap均高于单体组,但差异无统计学意义(P>0.05)。结论知母皂苷对芒果苷的体内吸收有一定的促进作用。
Objective To investigate the effects of timosaponin on absorption of mangiferin by in-vivo and in-vitro methods. Methods LC/MS/MS method was used to determine plasma mangiferin concentration in rats after intragastric administration of mangiferin or mangiferin mixed with timosaponin, and the main pharmacokinetic parameters were calculated. Intestinal absorption of mangiferin was studied by using everted gut sacs and by UPLC. Results Compared with mangiferin group, the plasma concentration, AUC and Cmax of mangiferin were significantly increased, and Tmax was prolonged to 3 h in mangiferin-timosaponin group. Q90, vt and Paup of mangiferin at ileac segment of rats in timosaponin-mangiferin （1 ： 1 ） group were higher than that in mangiferin monomer group（P 〉 0.05）. Conclusion Timosaponin can increase the absorption of mangiferin.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2012年第5期545-548,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省自然科学基金(8151040701000014)
广东省科技计划项目(2009B080701023)
广东省科技厅资助项目(2010B030700078)
关键词
知母皂苷
芒果苷
血药浓度
翻转肠囊
吸收
Timosaponin
Mangiferin
Plasma concentration
Everted gut sacs
Absorption
