^(60)Co-γ射线辐射对大白杜鹃的矮化效应

Effect of ^(60)Co-γRadiation on the Dwarfing Mechanism of Rhododendrondecorum

研究了不同剂量的60Co?γ射线辐射对大白杜鹃生长特性、叶片气孔特征及内源激素含量变化的影响,利用酶联免疫吸附测定法(ELISA)测定幼苗内源激素含量。结果表明:60Co?γ射线辐射对大白杜鹃幼苗的生长有不同程度的抑制作用,在20～60Gy范围内,辐射处理的苗高低于对照;辐射后叶片的气孔密度、大小与对照有显著差异,气孔开放面积减小;辐射处理后吲哚乙酸(IAA)、赤霉素(GA3)、玉米素核苷(ZR)含量降低,脱落酸(ABA)含量增加,较高水平的ABA含量有利于植株的矮化。

The effects of different levels of 60 Coγ-ray radiation on the growth,stomata characteristics and endogenous hormones of Rhododendron decorum were studied,the changes of endogenous hormones in seedlings were determined by ELISA.The results showed that 60 Coγ-ray radiation had different degrees of inhibition on the seedling growth of Rhododendron decorum,seedlings height radiated was lower than control in the rang of 40～60Gy;the stomatal density,size of radiated leaves were significant differences with the control,and the stomatal opening area was reduced.The contents of IAA,GA 3 and ZR were promoted while ABA was inhibited under the treatments of 60 Coγ-ray radiation,the higher content of ABA were good for the plants dwarfing.