兔眼蓝莓“灿烂”组织培养与植株再生研究

Tissue Culture and Plant Regeneration of Vacciniumashei‘Britewell’

以兔眼蓝莓"灿烂"(‘Britewell’)当年生带腋芽半木质化茎段为外植体,研究了不同培养基、激素对兔眼蓝莓"灿烂"植株再生的影响。结果表明:WPM为适宜基本培养基;WPM+(0.5～1.0)mg/L ZT组合是最适的初代幼芽诱导培养基,诱导率达86%以上;WPM+0.1mg/LTDZ+0.1mg/L IBA组合是较合适的增殖培养基,增殖率可达5.48,继代培养宜以较低浓度的TDZ(0.02mg/L和0.05mg/L)交替使用为好;1/2WPM+1.0mg/L 6-BA是较理想的生根培养基组合,有效生根率达63.2%。

Taking semi-lignified stem with axillary bud of Vaccinium ashei ‘Britewell’as explants,the effects of the different medias and hormones on plant regeneration were studied.The results showed that WPM was a suitable basic medium.The optimal primary medium for stem with axillary bud was WPM＋（0.5～1.0） mg/L ZT,and the induction rate was more than 86%;the suitable proliferation medium was WPM＋0.1mg/L TDZ＋0.1mg/L IBA,the proliferation rate up to 5.48times.Following the subculture should be at a lower concentration of TDZ（0.02mg/L and 0.05mg/L） alternating as well;the rooting medium was 1/2WPM＋1.0mg/L BA,and the effective rooting rate reached 63.2%.