生姜通过器官发生途径体外再生的研究

Study onin vitro Regeneration of ZingiberofficinaleRosc.by Organogenesis

以生姜茎尖为外植体,采用正交实验设计研究了不同激素组合和浓度的培养基对茎尖分化的影响。结果表明:茎尖经无菌处理,接种于MS+KT 2.0mg/L+NAA 1.0mg/L的培养基上,可使芽、根相继分化,形成的幼芽数平均为3.5个,继代培养历经60d可产生大量的带根苗,为生姜通过器官发生途径体外再生扩繁种苗提供了有效方法。

Taking vegetative buds of Zingiber officinale Rosc. as explants, its differentiation with different combinations and concentrations of plant hormone were studied by the method of orthogonal design. The results showed that the aseptic buds were cultured on MS medium supplemented with a combination of 2. 0 mg/L KT and 1.0 mg/L NAA gave the shoot and root differentiation,and the multiple shoot showed mean number of 3. 5. The repeated subculture resulted in rapid plantlets multiplication during 60 days. It was to provide an effective method for obtaining large scale planting material of ginger by the organogenesis pathway.