枣树幼叶总RNA提取方法的比较研究

Comparison of Four Methods for Extracting of Total RNA From Young Jujube Leaves

以枣树幼嫩叶片为试材,分别采用DEPC水法、SDS抽提法、CTAB-LiCl和异硫氰酸胍法4种方法进行总RNA的提取试验,并对其进行了紫外、变性琼脂糖凝胶电泳及RT-PCR检测。结果表明:4种提取方法中DEPC水法提取的总RNA质量最好,SDS抽提法和CTAB-LiCl法次之,异硫氰酸胍法效果最差。

Taking young jujube leaves as material,RNA in jujube leaves was extracted by the DEPC water method,SDS method,CTAB-LiC1 method and isothiocyanate guanidine method. The quality of the total RNA was tested by nuclear acid/protein analyzer, denaturation agarose gel electrophoresis and RT-PCR. The results showed that total RNA extracted by DEPC water method had the best quality and reliability, followed by SDS extraction method and CTAB-LiC1 method, the isothiocyanate guanidine method was the worst.