反复肺炎患儿血清β-防御素-2水平及临床意义

The expression and significance of human b-defenses 2 in serum of the children with recurrent pneumonia

目的:探讨血清中β-防御素-2(human β-defensins 2,hBD-2)在反复肺炎发病中的可能作用。方法:收集50例5～14岁反复肺炎和50例健康儿童的血清,处理后应用酶联免疫吸附法(ELISA)检测hBD-2和IL-8浓度,应用电阻法检测血液中性粒细胞比例,并分析hBD-2与IL-8、中性粒细胞比例之间的相关性。结果:(1)反复肺炎组和健康对照组hBD-2浓度比较差异无显著性(F=1.12,P=0.727)。(2)反复肺炎组IL-8浓度和中性粒细胞比例显著高于健康对照组,两组比较差异有显著性(F=1.25,P=0.00;F=1,P=0.00)。(3)反复肺炎组hBD-2与IL-8、中性粒细胞比例无相关性(r=0.02,P>0.05;r=0.13,P>0.05);IL-8与中性粒细胞比例呈正相关(r=0.62,P=0.00)。结论:反复肺炎患儿急性感染期血清hBD-2表达水平没有升高,提示呼吸道防御功能存在障碍,这可能是反复肺炎患儿呼吸道容易发生微生物感染的原因之一。

Objective To discuss the potential effection of hBD-2 in recurrent pneumonia. Method Collected 50 samples serum on 5-14 years old children with recurrent pneumonia and healthy children, and then tested hBD-2 and IL-8 concentration by ELISA, detected the percentage of neutrophils in blood by electric resistance method, and analyze the correlation between hBD-2, IL-8, and the percentage of neutrophils. Results （ 1 ） The concentration of human β-defenses 2 in patients was similar to that in control group （F = 1.12, P = 0.727）. （2）The expression of interleukin-8 and neutrophil in patients were significantly higher than those in control group （F = 1.25, P = 0.00; F = 1, P = 0.00）. （3） There was no correlation between the concentration of human p-defenses 2 and interleukin-8, neutrophil in patients （r = 0.02,P 〉 0.05; r = 0.13,P 〉 0.05）; It was positive correlation between the expression of interleukin-8 and neutrophil （r = 0.62, P = 0.00）. Conclusion The expression and level of human β-defenses 2 didn＇ t increase in the children with acute recurrent pneumonia, showing disturbance of the defensive ability of respiratory tract, which may be the reason of microorganism infection of respiratory tract easily occurring in the children with recurrent pneumonia.