碲化镉量子点对小鼠肝、肾的毒性研究

Study on the hepatotoxicity and nephrotoxicity of mice induced by CdTe QDs

目的探讨碲化镉量子点(CdTe QDs)对小鼠肝、肾的毒性作用。方法将40只雄性ICR小鼠随机分为5组,每组8只,采用尾静脉注射方式染毒。染毒浓度分别为0(对照组)、0.5、5、50和500 nmol/ml CdTe QDs溶液,每只动物注射0.2 ml,对照组注射等体积的生理盐水,染毒24 h后小鼠脱臼处死。取肝脏、肾脏秤重,计算脏器系数;测定小鼠血清生化和血液常规指标,并对肝脏和肾脏进行病理组织学检查。结果各染毒浓度组肝、肾脏器系数与对照组比较,差异无统计学意义(P>0.05);肌酐(CRE)和血小板(PLT)与对照组比较显著降低(P<0.01);各染毒浓度组动物的肝细胞可见不同程度的水样变、肝细胞肿胀、肝细胞点状坏死,伴淋巴细胞浸润;肾小管浊肿、肾小动脉扩张充血及间质部分区域小血管充血。结论本研究条件下,CdTe QDs能够对小鼠肝、肾产生毒性作用。

Objective To investigate the effects of hepatotoxicity and nephrotoxicity on mice indcced by CdTe QDs. Methods Forty ICR male mice were randomly divided into 5 groups：one control（ normal saline）group,four CdTe QDs groups （exposed by intravenous injection of 0.2ml of CdTe QDs at the concentrations of 0,0.5,5,50 and 500 nmol/ml respectively）. All mice were decapitated 24 h after the injection, the ratios of liver and kidney to body weight, the pathological changes of liver and kidney, serum biochemically indexes and hematology indexes were detected. Results The ratios of liver and kidney to body weight in experimental groups were no significant difference in comparison to the control（P 〉0. 05）. The CRE level in serum of mice and the PLT of hematology in the exposure groups were all decreased than those of the control group（P 〈0.01 ）. Pathologic examination showed that that compared with the control, along with the increase of the dosage in the liver tissue, the hydmpic degeneration of hepatocyte around the central vein, hepatocyte disorder, punctatenecrosis of some hepatocytes were found. It was also observed that the renal tubule serious swelling and hyperemia. Conclusion CdTe QDs could induce hepatotoxieity and nephrotoxicity of the mice in the condition of this experiment.