NDRG1和相关蛋白在乳腺癌中的表达及相关性分析

Expression of NDRG1 and associated proteins in breast cancer and their correlation

目的探讨N-myc下游调节基因1(NDRG1)在乳腺癌组织中的表达情况及其与ER、PR、突变型p53、c-erb-B2表达的相关性。方法采用免疫组化SP法分别检测82例乳腺癌组织中NDRG1、ER、PR、突变型p53及c-erb-B2表达情况,并行相关性分析;分析NDRG1蛋白表达与临床病理特征的关系。结果早期乳腺癌患者NDRG1呈高表达,晚期乳腺癌患者NDRG1低表达(P=0.014);不同的病理类型之间NDRG1表达无明显相关性;NDRG1表达与突变型p53表达呈负相关(r=-0.233,P=0.035),与ER、PR和c-erb-B2表达无关(P>0.05)。结论 NDRG1可能在乳腺癌的发生、发展中发挥重要的抑制作用,可作为乳腺癌基因研究和治疗的靶点。

Objective To investigate the expression of N-myc down stream regulated gene 1 （NDRG1） in breast carcinoma tissues and the relations with ER, PR, p53, c-erb-B2. Methods The expressions of NDRG1 ,ER,PR, p53 and c-erb-B2 proteins were detected in 82 breast carcinoma samples by immunohistoehemieal staining, then the correlation of their expression was analyzed. The relationship between clinical characteristics and NDRG1 expression was also studied. Results The positive rate of NDRG1 protein expression was higher in early stage breast cancer than that in advanced breast cancer（ P = 0. 014）. NDRG1 protein expression has sig- nificantly negative correlation with p53 （ r = - 0. 233, P = 0. 035 ） , but no association could be detected among NDRG1 , ER, PR and e-erb-B2 （ P 〉 0. 05 ）. Conclusion NDRG1 plays an important inhibition role in the development of breast cancer, and it may be con- sidered as a valuabe marker for the gene research and therapy of breast carcinoma.