MMP13在非小细胞肺癌组织中的表达及其预后价值

Expression and prognostic value of MMP13 in non-small cell lung cancer

目的探讨MMP13在非小细胞肺癌(NSCLC)组织中的表达及其与浸润、转移和预后的关系。方法采用免疫组化法检测87例NSCLC组织和30例癌旁正常组织中MMP13蛋白的表达水平。分析MMP13表达与NSCLC临床病理特征的关系,应用Kaplan-Meier法和多因素Cox模型对87例NSCLC患者的生存进行分析。结果 MMP13在NSCLC组织和癌旁正常组织中的阳性表达率分别为70.1%(61/87)和6.7%(2/30),差异具有统计学意义(P<0.001)。MMP13表达与T分期、淋巴结转移和TNM分期有关(P<0.05)。Kaplan-Meier法生存分析曲线显示,MMP13阳性表达组患者的5年生存率为18.4%,显著低于阴性表达组的52.1%(P=0.001),同时TNM分期(P<0.001)、淋巴结转移(P<0.001)和T分期(P=0.002)均可影响生存期。Cox模型多因素分析显示,仅MMP13表达(P=0.042)和TNM分期(P=0.001)是影响肺癌预后的独立因素。结论 MMP13与肺癌的浸润和转移密切相关,可能是肺癌预后判断的一项指标。

Objective To explore the expression of MMP13 and its relationship with invasion, metastasis and prognosis of non-small cell lung cancer（NSCLC）. Methods The expression of MMP13 was detected by immunohistochemistry in 87 specimens of NSCLC tissues and 30 specimens of adjacent normal tissue. X2 test was applied to analyze the relationship between expression and clini- copathologic characteristics. Kaplan-Meier univariate and multivariate Cox model were applied to analyze the survival of NSCLC. Re- sults The positive expression rates of MMP13 in NSCLC and adjacent normal lung tissues were 70. 1% （ 61/87 ） and 6. 7% （ 2/30 ） with significant differences（P 〈 0. 001 ）. The expression of MMPI3 was associated with T stages, lymphatic metastasis and TNM stages （P 〈 0. 05 ）. Kaplan-Meier univariate analysis revealed the five-year survival rate of MMP13-positive patients was lower than that of MMP13-negative patients （ 18.4% vs. 52. 1%, P = 0. 001 ）. Meanwhile, TNM stages （P 〈 0. 001 ）, lymphatic metastasis （P 〈 0. 001 ） and T stages （ P = 0. 002 ） could influnce the survival time of patients with NSCLC. Cox multivariate survival analysis revealed only MMP13 expression （ P = 0. 042） and TNM stages （ P = 0. 001 ） as independent prognostic factors. Conclusion MMP13 is closely corre- lated with invasion and metastasis of lung cancer, and its positive expression may suggest poor prognosis in NSCLC patients.