超临界流体萃取野菊花中绿原酸工艺研究

Extraction Process of Chlorogenic Acid from Wild Chrysanthemum Flower via Supercritical Fluid Extraction

对超临界流体萃取法提取野菊花中绿原酸的工艺进行了研究,探讨了超临界萃取过程中萃取压强、萃取时间、萃取温度对绿原酸提取率的影响,并通过正交试验对工艺条件进行优化,采用紫外分光光度法测定提取液中绿原酸的含量。结果表明,最佳工艺条件为,水为助提剂、萃取压强25 MPa、萃取时间3 h、萃取温度50℃,可以获得较高的得率0.488 3%。

Researched on the process of the use of supercritical fluid extraction technique to extract chlorogenic acid from wild chrysanthemum flower. The paper discussed the factors affecting the yield of chlorogenic acid by supereritical fluid extraction technique, including pressure, time and temperature. Orthogonal experiment was used to optimize the process and UV speetrophotometry was used to determine the amount of chlorogenic acid in the extract. The result showed that the optimal process conditions were water as the chemical additives, pressure 25 MPa, 3 hours of extraction time and temperature 50 ~C. This obtained a relatively high yield of 0.488 3 %.