草鱼酶解条件的研究

Conditions of Hydrolyzing Grass Carp by Enzymes

采用木瓜蛋白酶水解草鱼鱼蛋白,采用单因素试验考察各因素对水解效果的影响,通过正交试验确定水解的最佳条件。实验结果表明,木瓜蛋白酶水解草鱼的最佳条件是:酶浓度为2.0%,底物浓度为3.0%,酶解时间为4 h,pH为7.0,酶解温度为60℃,在此条件下草鱼的水解度可达到45.93%。

The protein of grass carp were Hydrolysis by Papaya. The effect factors of hydrolysis were investigated, and the best conditions were decided by orthogonal experiment. According to the result, the best hydrolysis conditions were enzyme concentration 2.0 %, substrate concentration 3.0 %, time 4 h, pH 7.0, temperature at 60 C, in which the hydrolysis of grass carp will get 45.93 % fulfilled.