不育小白鼠睾丸转铁蛋白的定量分析

Quantitative analysis of transferrin in relation to male infertility in Kunming mice

目的采用FQ-PCR扩增不育小白鼠睾丸转铁蛋白基因,探讨腺嘌呤致昆明小白鼠雄性不育的可能机制。方法用含0.5%的腺嘌呤饲料饲喂小白鼠5～10d,分别在实验第5、10d取睾丸组织,常规方法切片,HE染色后观察病理变化。根据转铁蛋白基因序列设计、合成引物,荧光定量PCR检测实验组与对照组转铁蛋白基因表达水平。结果第5、10d实验组小白鼠睾丸组织Tf基因水平差异有统计学意义(t=5.178,P<0.01);对照组5周末Tf基因表达量是实验组的2.51倍(t=2.783,P<0.05),第10周的表达量是实验组的6.59倍(t=11.864,P<0.01)。结论腺嘌呤致雄性不育小鼠睾丸转铁蛋白基因表达水平降低。转铁蛋白基因可作为不育症诊断及治疗的重要靶标。

Objectives To use FQ-PCR to amplify the transferrin（Tf） gene in infertile male Kunming mice and to study the mechanism of adenine-induced male infertility.Methods Adenine-induced male infertility was produced in mice by providing them with a 0.5% adenine diet.Testicular tissue was sampled on days 5 and 10 and pathologic changes were observed after HE staining.Primers were designed on the basis of the Tf sequence.Fluorescence quantitative PCR was used to measure differences in the level of expression in the control group and experimental group.Results There were significant differences（t=5.178,P〈0.01） in Tf gene expression in experimental mice on days 5 and 10.The level of expression in the control group was 2.51 times（t=2.783,P〈0.05） that in the experimental group at the end of week 5.The level of expression was 6.59 times（t=11.864,P〈0.01）that in the experimental group in week 10.Conclusion Adenine-induced male sterility reduced the level of expression of the transferrin gene in mouse testes.The Tf gene can serve as a gene target for male infertility therapy.