芪竹方中单体及配伍对人胃癌细胞MGC-803抑制作用研究

Inhibition of Single Drug and Combined Drugs in Qizhu Decoction to the Proliferation of Human Gastric Cancer Cell MGC-803

目的:观察芪竹方单体成分薯蓣皂苷、莪术醇及两者配伍对人胃癌细胞MGC-803的增殖抑制作用,初步探讨芪竹方复方在抗肿瘤治疗中的有效成分及作用机制。方法:选用5个剂量的薯蓣皂苷、莪术醇单药组,及2个剂量的两药配伍组在24h、48h 2个不同时间点作用人胃癌细胞MGC-803,采用MTT法检测药物对MGC-803的增殖抑制率。结果:一定浓度范围内,薯蓣皂苷及莪术醇均对MGC-803细胞的增殖有剂量、时间依赖性抑制作用,且两药配伍使用较单药能产生更好的抑制作用。结论:薯蓣皂苷及莪术醇可能为芪竹方抑制人胃癌细胞MGC-803增殖的有效成分。

Objective ：To observe the inhibition of curcumol and dioscin and their combination from Qizhu decoction to the proliferation of human gastric cancer cell MGC - 803, and to investigate the active principles and anti - tumor mecha- nisms of Qizhu decoction. Methods ： MGC - 803 was treated with five dosages of curcumol and dioscin single and two dosages of the combination of curcumol and dioscin for two times at the points of 24h and 48h, and the inhibitory rate of the drugs on MGC -803 proliferation was detected by MTr assay. Results：Within a certain concentration range, both curcumol dioscin have the time - and - dose - dependent inhibitional effect on the proliferation of MGC - 803, and using the combination of curcumol and dioscin has a better effect than using single of the two drugs. Conclusions：Curcumol and dioscin from the Qizhu decoction might be the active principles in inhibiting the proliferation of MGC -803.