一起聚集性肺炎疫情病原的快速实验室诊断与分析

Rapid Laboratory Diagnosis of a Pneumonia Cluster

目的:通过实验室病原学检测,结合流行病学史及临床表现,初步筛查疫情致病病原体。方法:采集患者咽拭子标本,分别采用荧光PCR方法检测甲型流感病毒、乙型流感病毒、肺炎支原体基因。结果:11份患者咽拭子中有4份标本荧光PCR扩增支原体基因阳性,1份为弱阳性。所有标本的甲、乙型流感荧光PCR扩增结果为阴性。同时采集与患者同班的3例无症状对照标本,3例标本的肺炎支原体DNA检测均为阴性。结论:实验室研究结果并结合此次疫情的流行病学和患儿的临床表现,初步判断可能是一起由肺炎支原体引起的聚集性支原体肺炎疫情。

Objective：To primarily determine the pathogen responsible for a pneumonia cluster among students in a primary school in Nanjing, based on laboratory pathogen detection, epidemic evidence and clinical symptoms.Method：Realtime fluorescent polymerase chain reaction were performed to examine nosethroat swab samples collected from patients for Mycoplasma pneumoniae, influenza A and B.Resuit：The realtime PCR results were positive（including weak positive） for Mycoplasma pneumoniae（MP） in 4 out of 11 samples and negative for influenza A and B in all 11 samples. Conclusion：Combined with clinical symptoms and epidemic evidence, we primarily concluded that this is a pneumonia cluster caused by mycoplasma pneumoniae.