摘要
目的建立高效液相色谱法测定止咳类中药制剂中添加成分磺胺甲恶唑含量,以更好地控制中成药及保健食品的质量。方法色谱柱:Agilent Zorbax C18柱(4.6 mm×250 mm,5μm);流动相A为乙腈-甲醇(1∶1),流动相B为甲酸铵溶液(取甲酸铵0.3 g,加水1 000 mL溶解),进行梯度洗脱;流速:1.0 mL.min-1;检测波长:225 nm。结果磺胺甲恶唑在20.29～202.89μg.mL-1范围内浓度与峰面积呈良好的线性关系(r=0.999 6),平均回收率为99.91%,RSD=0.86%。结论本法准确、分离度好、回收率高、专属性强,可作为止咳类中药制剂中添加成分磺胺甲恶唑的检测方法。
Objective To establish an HPLC method for the determination of sulfamethoxazole added in Chinese herbal cough preparations for better quality control of traditional Chinese medicines and health food. Methods Agilent ZORBAX C18 （4.6 mm×250 mm ,5 μm） was adopted with mobile phase A of acetonitfile -methanol（ 1：1 ） and B of ammonium formate solu- tion（0. 3 g ammonium formate dissolved in 1 000 mL water） by gradient elution. The flow rate was 1.0 mL .min-1 and the detec- tion wavelength was 225 nm. Results The linear range of sulfamethoxazole was 20.29 - 202.89 μg . mL - 1（ r = 0. 999 6 ）. The av- erage recovery was 99.91% with RSD =0. 86%. Conclusion The method was accurate with high resolution,high recovery rate and good specificity,and it can be used for the determination of sulfamethoxazole added in Chinese herbal cough preparations.
出处
《齐鲁药事》
2012年第9期509-511,530,共4页
qilu pharmaceutical affairs
