高效液相色谱法测定百菌清

HPLC Analysis of Chlorothalonil

文章建立了百菌清高效液相分析方法。实验结果表明,该方法测定百菌清在158.40～792.00μg/mL范围内有良好的线性关系,相关系数R2=0.9997,最小检测限为2.01×10-10 g,相对标准偏差为0.054%,加标回收率在99.0%～99.8%之间,平均回收率为99.3%。本方法使用C18 SB反相柱,以甲醇/二氧六环(98/2,V/V)为流动相,在检测波长250 nm下进行定量分析。

The paper set up a HPLC method for analyzing chlorothalonil.As the results showed,the linear regression coefficient of the method was 0.9997 and the limits of the detection were 2.01×10-10g.The relative standard deviation was 0.054 %.The recoveries were in the range of 99.0 %～99.8 %,and the average recovery was 99.3 %.The quantitative method was conducted by using C18 SB reversed-phase column.The mobile phase was methanol/1,4-Dioxane（98/2,V/V）,and it was detected at 250 nm with VWD.