摘要
目的:研究小鼠弥散性小栓塞模型的认知功能变化。方法:40只小鼠随机分为实验组和对照组各20只,实验组通过颈外动脉注射胆固醇微栓子建立小鼠弥散性小栓塞模型,对照组注射生理盐水。通过免疫荧光染色观察实验组脑片。利用旷野实验、新物再认实验、恐惧条件化测试及水迷宫实验观察小鼠认知功能变化。结果:手术后第4天,实验组脑内可观察到散在的小梗死灶,梗死灶主要位于损伤侧脑半球内,并可见局灶性神经元丢失。旷野实验中实验组未见明显运动功能受损及焦虑行为。新物再认实验中实验组在手术后第1、2周可见新物再认功能受损。恐惧条件化测试可见在手术后第2周,实验组可有海马依赖性认知功能损害,手术后28天水迷宫测试显示实验组存在空间探索功能受损。结论:小鼠脑内弥散性小栓塞可引起小鼠局灶性神经元丢失,并导致小鼠轻度认知功能受损。
Objective： To explore the effects of diffuse micro-strokes on murine cognitive function.Methods： Micro emboli made of cholesterol crystal was injected into the external carotid artery in mice to induce diffuse micro infarcts.Immunohistochemistry was performed to study the changes of brain in this model.We also used a novel object recognition test,open field test,fear conditioning test and water maze test to evaluate changes in the cognitive function.Results：Diffuse micro infarcts were found in the injured hemispheres.The localized loss of neurons were observed in the lesion sites.The novel objected recognition test showed the diffuse micro stroke mice had impaired cognitive function in the first two weeks post surgery while the open field test showed that they had no motor function impairment and anxiety.The fear conditioning test showed the mice had cognitive impairment two weeks post stroke.The water maze test showed the mice had a significant cognition impairment in spatial probe test.Conclusion： The diffuse micro infarcts in mice brain can cause localized loss of neurons and result in mice cognitive impairment.
出处
《神经损伤与功能重建》
2012年第5期313-317,379,共6页
Neural Injury and Functional Reconstruction
