改善内皮细胞在聚酯膜上黏附和活性的实验研究

Experimental study on promotion of endothelial cells adhesion and activity on polyester fibers

目的研究使用纤维黏连蛋白(fibronectin,FN)是否能加速内皮细胞在聚酯膜上的黏附和细胞活性。方法选用猪髋动脉内皮细胞(PIEC)作为细胞模型,应用同位素3H与胸腺嘧啶结合的方法标记细胞,通过检测放射性计算出在共培养不同时段黏附于聚酯膜上的细胞数,利用罗丹明123荧光染色显示黏附细胞中的活性细胞数量,并采用扫描电镜观察黏附细胞形态及内皮化程度。结果共培养24h后FN组黏附细胞数与对照组及明胶组相比明显增多(P<0.001),且活性最强,内皮化程度最高,72 h后明胶组与FN组细胞黏附程度相近(P>0.05),但明胶仅增加黏附细胞数,对于内皮细胞功能无促进作用,而未包被任何材料的对照组细胞较慢,黏附较少,内皮化进程慢。结论 FN可在短时间内促进内皮细胞黏附于聚酯膜上并具有很强的活性。

Objective To evaluate the effect of FN endothelial cells adhere to and grow on the surface of polyester fiber membrane. Methods Porcine iliac artery endothelial cells （PIECs） were chosen as cell model, PIECs were labeled with isotope through the combination of 3 H-thy- midine, the radioactivity of cells was used to determine the number of attached cells to the ma- terial surface following the time course. Rhodamine123 was used to represent the activity of ad- hesive cells. The cell morphologies and process of endothelialisation were observed by scanning electron microscope. Results FN coating had significant impact on cell adhesion compared with control and gelatin after 24 h （ P 〈 0. 001 ）, and had high cell activity and endothelialisati- on degree showed on immunofluorescence and scanning electron microscope data. There was no significant difference between FN coating and gelatin coating in attached cells after 72 h （P 〉 0.05 ）, but the effect of gelatin on endothelialisation was weak. In contrast, the cells of controlgroup without nothing coating had low abilities of adhesion, and the process of endothelialisati- on was bogged down. Conclusion FN has potential to promote endothelial cells adhesion on polyester fibers in a short period and has high activity.