摘要
目的:研究二氢杨梅素体外对醛糖还原酶(AR)的影响。方法:取正常SD大鼠晶状体制备醛糖还原酶,以依帕司他1×10-7mol/L为阳性对照,观察二氢杨梅素1.0×10-5、4.0×10-5、1.4×10-4、6.4×10-4mol/L四个浓度体外对醛糖还原酶的抑制作用。结果:随着二氢杨梅素浓度的增加,醛糖还原酶的活性逐渐降低,且呈一定的剂量-效应关系,在终浓度为6.4×10-4mol/L时,其对醛糖还原酶活性的抑制率为54.3%。结论:二氢杨梅素在体外有抑制醛糖还原酶的作用。
Objective: To study the effect of dihydromyricetin on the activity of aldose reductase (AR) in vitro. Methods: Aldose reductase was ex- tracted from lens of normal SD rat. Epalretat ( 1×10^-7 mol/L) was as a positive. The inhibitory effects of dihydromyricetin, at a concentra- tion of 1.0 × 10^-5 , 4.0 × 10^-5 , 1.4 ×10^4 and 6.4 ×10^4 tool/L, on the activity of aldose reductase were investigated in vitro. Results: Di- hydromyricetin markedly inhibited the activity of aldose reductase, in a concentration-dependent manner, with the inhibitory ratio of 54.3% at the concentration of 6.4×10^-4 mol/L. Conclusion: Dihydromyricetin has the inhibitory activity on aldose reduc'tase in vitro.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2012年第4期43-45,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家自然科学基金资助项目(30760306)
云南省应用基础研究计划重点项目(2006C009Z)
关键词
醛糖还原酶
二氢杨梅素
糖尿病
dihydromyricetin (二氢杨梅素)
aldose reductase
diabetes