晚期小鼠肺癌模型对益气养阴清热方及化疗反应的研究

Study on establishing mice model of advanced lung cancer bearing Lewis lung carcinoma

目的:建立晚期肺癌的动物模型,研究其对化疗药物及益气养阴清热方的治疗反应状况,寻找适合评价以控制症状、改善生活质量为目的疗法的实验模型。方法:通过胸腔移植的方法建立晚期肺癌的动物模型,研究该模型的生存期及对氟尿嘧啶(20mg/kg)、顺铂(3mg/kg)、益气养阴清热方浓缩中药溶液(28.6mg/kg)的治疗反应状况,用免疫组化的方法检测了肿瘤组织内表皮生长因子(EGF)和表皮生长因子受体(EGFR)等蛋白表达(400×),分析了该模型较皮下移植模型的优势。结果:经过治疗后,胸腔组内的中药组、顺铂组体重下降程度较对照组和氟尿嘧啶组体重下降程度低(P<0.05)。皮下组内各小组体重变化无明显差异。胸腔组较相应皮下组饲料消耗量少。胸腔组内的对照组和氟尿嘧啶组饲料消耗量较其他组明显减少(P<0.05),益气养阴清热方组、顺铂组间无明显差异。皮下组内各小组饲料消耗量无明显差异。对照组和氟尿嘧啶组中EGF、EGFR阳性细胞数较其他组明显增多。结论:胸腔移植模型可作为晚期肺癌动物模型,胸腔移植模型较皮下移植模型能更好地反映药物的作用,化疗药物、益气养阴清热方对EGF、EGFR等的表达有不同程度的抑制作用,益气养阴清热方对抑制肿瘤发展优势不明显,但对改善模型生存时间、生活状态有较好的作用。

Objective： To find a nice animal model for evaluating the therapy of controlling the symptoms and improving the quality of life. Meth- ods： （ I ） Model： The C5 BL/6 mice were divided into two groups： subcutaneous implant group and thoracic cavity implant group. The sub- culturing Lewis lung cancer cells were implanted into thoracic cavity or armpit of mice to make models. （2） Group： Subcutaneous implant group and thoracic cavity implant group were respectively divided into five groups randomly： ~＇~k=]~ ~ group, DDP group and control group. Each group contained twenty mice. Four drugs were injected into four chemotherapy groups on the 6th day and the 11 th day of building mod- els respectively. （4） Quality of Life ： The weight of the mice and the consumption of feed were measured at 8：00-9：00 p.m. everyday. The expressions of EGF and EGFR in the lung cancer tissue were examined by immunohistochemical method. Results： （ 1 ） Quality of life ： （~ Weight： The weight of thoracic cavity implant group was significantly lower than that of subcutaneous implant group. In thoracic cavity im- plant group, the weight of control group and of ~p＇~l^ll）~ group were both obviously lower than those of other groups （P 〈0.05）. But there were no significantly differences in the weight of subcutaneous implant group. （~）Consumption of feed ： The feed consumption of thoracic cavity implant group was significantly less than that of subcutaneous implant group. In thoracic cavity implant group, the feed consumptions of the control group and of ~/~II~ group were both apparently less than those of other groups （P 〈 0.05 ）. But there were no significantly differ- ences in the weight of subcutaneous implant group. （2） IHC： The expression of EGF and of EGFR in DDP group were both significantly less than those in other groups （ P 〈 0.05 ）. Conclusions： Thoracic cavity implant model in responding to the therapeutic efficacy was superior to subcutaneous implant model. Different drugs inhibited the expression of EGR and EGFR. Traditional Chinese medicine had no obvious advantage supressing the development of tumour, but it could prolongate survival time and improve life in our model.