6种水产常用抗生素对鳗弧菌的抗菌后效应研究

Postantibiotic effects of 6 kinds of antibiotics on bacterium Vibrio anguillarum

为了解水产养殖业常用抗生素对鳗弧菌的抗菌后效应,本研究采用试管二倍稀释法测定乳酸恩诺沙星、乳酸诺氟沙星、盐酸沙拉沙星等6种抗生素对鳗弧菌的最小抑菌浓度(MIC);采用菌落计数法测定6种抗生素的抗菌后效应(PAE)。结果显示,6种抗生素在1、2、4倍MIC时对鳗弧菌的PAE分别为:0.17±0.11,1.45±0.21,1.81±0.33(乳酸恩诺沙星);0.54±0.11,0.72±0.12,1.17±0.22(乳酸诺氟沙星);0.52±0.19,0.72±0.25,1.13±0.22(盐酸沙拉沙星);0.46±0.16,0.86±0.16,1.88±0.30(盐酸二氟沙星);0.68±0.21,1.42±0.34,1.91±0.20(盐酸恩诺沙星);0.67±0.13,0.79±0.12,1.25±0.17(烟酸诺氟沙星)。6种抗生素对鳗弧菌均表现出不同程度的抗生素后效应,并随着抗生素浓度的增加,PAE的时间也延长,呈药物剂量依赖模式。

The minimum inhibitory concentration （MIC） of 6 kinds of antibiotics against bacterium Vibrio anguillarum were determined by two-fold broth macrodilution method, and the postantibiotic effects （PAE） of 6 kinds of antibiotics were determined by colony counting. It was shown that at the concentrations of 1MIC, 2MIC and 4MIC, the PAE were 0.17 ± 0.11, 1.45 ± 0.21, 1.81 ± 0.33 （Enrofloxacin lactate）; 0.54 ± 0.11, 0.72 ± 0.12, 1.17 ± 0.22 （Norfioxacin lactate）; 0.52 -＋ 0.19, 0.72 ± 0.25, 1.13 ± 0.22 （Sarafioxacin hydrochloride）; 0.46 ± 0.16,0.86 ± 0.16,1.88 ± 0.30 （Difloxacin hydrocbloride）; 0.68 ± 0.21, 1.42 ± 0.34, 1.91 ± 0.20 （Enrofloxacin hydrochloride）; 0.67 ± 0.13, 0.79 ± 0.12, 1.25 ± 0.17 （Norfloxacin nicotinic）. The PAE of 6 kinds of antibiotics against bacterium V. anguillamm were in a time- and concentration-dependent manner.