耐热石油烃降解混合菌的筛选及其群落结构分析

Screening of a hydrocarbon-degrading bacterial group of heat-resistant and analysis of its community structure

对克拉玛依采集的部分石油污染土壤进行了筛选,得到了5组石油烃高效降解混合菌,其中混合菌KL9-1在45℃的条件下,通过7 d的降解,稀油的降解率达到43.27%,稠油的降解率达到20.09%。混合菌KL9-1经过多次分离纯化后,获得3株具有石油烃降解能力的优势单菌,3株单菌对稀油的降解率都在30%以上。结合分离单菌株的形态、生理生化特征和16S rDNA基因序列的分析结果,初步鉴定KL9-1-1为Pseudomonas putida,KL9-1-2和KL9-1-3为Pseudomonas sp.。

5 highly efficient hydrocarbon-degrading mixed bacteria were obtained from the petroleum-contaminated soil samples of Karamay by the traditional method of enrichment and acclimation. The KL9-1 group has a wide temperature tolerance range and higher hydrocarbon degrading ability. The degradation rate of thin oil and heavy oil was up to 43.27% and 20.09% respectively through 7 d at 45 ℃. After several times of isolation and purification, three dominant strains with the capability of hydrocarbon-degrading were obtained, and their petroleum degradation rates were all over 30%. According to its morphological, physiological, biochemical, and 16S rDNA sequence characteristics, the KL9-1-1 strain was identified as Pseudomonas putida, the KL9-1-2 strain and KL9-1-3 strain were identified as Pseudomonas sp.