促酰化蛋白基因301T>C多态性与新疆维吾尔族冠心病及血脂的关系

Association of the 301T>C of acylation stimulating protein gene polymorphism with coronary heart disease in Uygur patients in Xinjiang

目的探讨促酰化蛋白(acylation stimulating protein,ASP)基因301T>C多态性与新疆地区维吾尔族冠心病及血脂的关系。方法 385例冠心病患者(冠心病组)与483例冠状动脉造影结果阴性患者(对照组),2组采用聚合酶链反应-限制性片段长度多态性技术检测301T>C多态性,并检测其血脂水平。结果冠心病组ASP基因301T>C的3种基因型分布频率分别为TT型44.7%、CT型43.1%、CC型12.2%;对照组分别为TT型53.8%、CT型40.2%、CC型6.0%,2组基因型分布差异有统计学意义(P<0.01);Logistics回归分析结果显示,CC基因型个体患冠心病风险是TT基因型的0.784倍(OR=2.189,95%CI:1.251～3.830);冠心病组301T>C基因型亚组间三酰甘油水平差异有统计学意义(P<0.05);CC基因型三酰甘油水平高于TT基因型(P<0.05)。结论 ASP基因301T>C多态性与新疆维吾尔族人群冠心病的发生有一定相关性,CC基因型可能是冠心病发生的危险因素之一。

Objective To investigate the association of the 301T〉C polymorphism of acylation stimulating protein （ASP） gene with coronary heart disease （CHD） and the influence of serum lipid levels in Uygur patients in Xinjiang. Methods The polymorphisms of gene in 385 patients with CHD （CHD group） and 483 patients with negative coronary angiography results （control group） were analyzed with PCR restriction fragment length polymorphism, and the serum lipid level was detected as well. Results The frequencies of TT, CT and CC genotype were 44.7%, 43.1% and 12.2 % in CHD group, and were 53.8%, 40.2% and 6.0% in control group, which showed significant differences between two groups （P〈0.01）. Logistic regression analysis revealed that the risk of CHD was 0. 784 times higher in individuals carrying CC genotype than that in individuals carrying TT genotype （OR=2. 189, 95%CI： 1. 251 to 3. 830）. There was a significant difference in the level of serum triacylglycerol in different genotypes in CHD group （P〈0.05）, and serum triacylglycerol level was higher in patients carrying CC genotype than that in patients carrying TT genotype （P〈0.05）. Conclusion The 301T〉C polymorphism of ASP gene is correlated with the development of CHD, and C allele might be one of the risk factors of CHD.