畜禽用凝结芽孢杆菌液体菌剂杂菌控制及保存期初步研究

Preliminary Researches on Control of Miscellaneous Microorganisms and Preservative Period for Liquid Agent of Bacillus coagulan Applied to Animal

采用在凝结芽孢杆菌发酵液中添加不同防腐剂并结合调节pH值的措施,对畜禽用凝结芽孢杆菌液体制剂杂菌控制及保存期进行了初步研究。结果表明:在凝结芽孢杆菌发酵液中添加0.5%苯甲酸钠,调节发酵液pH值为4.0,并置阴凉干燥处常温保存时,可有效控制杂菌污染。在此条件下,凝结芽孢杆菌液体制剂保存30 d菌体存活率达约80%,保存180 d菌体存活率大于50%。

Combined application of adding different preservatives to fermentation broth of Bacillus coagulan and controlling pH value of fermentation broth, the control of miscellaneous microorganisms and preservation period of liquid agent of Bacillus coagulan applied to animal were studied preliminarily. The results showed that the contamination of miscellaneous microorganisms can be controlled when adding 0.5% sodium benzoate to fermentation broth, adjusting the pH value of fermentation broth to 4.0 and keeping fermentation broth preserved at conditions of dry, cool and normal temperature. Under these conditions, the survival rates of Bacillus coagulan in fermentation broth were respectively about 80% at 30 d and beyond 50% at 180 d.