桑青枯雷尔氏菌MR111的16S rDNA基因序列测定和系统进化分析

Sequencing and Phylogenetic Analysis of the 16S rDNA of Ralstonia solanacearum MR111 in Mulberry

对从发病的桑树中分离出的菌株MR111的16S rDNA进行PCR扩增和序列分析。结果表明:对菌株MR111进行16SrDNA的PCR扩增,获得了1 435 bp 16S rDNA序列。NCBI在线BLAST分析表明,该序列与茄科雷尔氏菌PC-3、RSGC-1等序列的同源性达95%。对28个茄科雷尔氏菌菌株的16S rDNA序列进行系统进化分析,MR111与来自空心菜(GY0501)、番茄(ZCz-3)等的菌株聚合在一个簇群,证实该菌株为青枯雷尔氏菌。命名为桑青枯雷尔氏菌MR111a。

PCR amplification and sequence analysis was applied to 16S rDNA of strain MR111 isolated from diseased mulberry. The results showed that the 1 435 bp of I6S rDNA sequence was obtained from PCR amplification. The resuhs of BLAST on NCBI indicated that the 16S rDNA has 95% identity with Rcdstonia solanacearum strain PC-3 and RSGC-1 （GenBank accession number： HM992931 and JQ320374）. The results of phylogenetic analysis based on the 16S rDNA from 28 Ralstonia solanacearum strains showed that MR111 is in the same branch with GY0501 from Ipomoea aquatica and ZCz-3 from Lycopersicon esculentum. Therefore, the MR111 is identified as Ralstonia solamwearum and named as MR111a.