草分枝杆菌F.U.36注射液预防过敏性紫癜复发效果及其对免疫功能的影响

Effectiveness of Mycobacterium Phlei Injection in Preventing the Recurrence of Henoch-Schonlein Purpura and Its Effects on Immunological Functions

目的探讨草分枝杆菌F.U.36注射液预防过敏性紫癜复发的疗效及其对免疫功能的影响。方法选取2005年1月—2010年12月在我院儿科住院的过敏性紫癜患儿76例为研究对象,随机数字表法分为草分枝杆菌组(38例)和一般治疗组(38例),一般治疗组给予常规治疗,草分枝杆菌组在常规治疗的基础上加用草分枝杆菌F.U.36注射液肌肉注射,观察两组治疗后6个月和1年内的复发情况,同时检测治疗前后外周血免疫球蛋白(Ig)G、IgA、IgM水平及T淋巴细胞亚群CD3、CD4、CD8、CD4/CD8。另取15例正常儿童作为对照组。采用SPSS 13.0统计软件进行统计学处理。结果草分枝杆菌组在治疗后6个月及1年内复发率均低于一般治疗组,差异有统计学意义(P<0.05)。治疗前,草分枝杆菌组、一般治疗组和对照组血清IgA水平及CD3、CD4、CD4/CD8比较,差异均有统计学意义(P<0.05);草分枝杆菌组和一般治疗组较对照组IgA水平明显升高,CD3、CD4、CD4/CD8明显降低,差异均有统计学意义(P<0.05);草分枝杆菌组和一般治疗组IgG、IgA、IgM水平及CD3、CD4、CD8、CD4/CD8比较,差异均无统计学意义(P>0.05)。治疗后,草分枝杆菌组较一般治疗组IgA水平明显降低,CD3、CD4、CD4/CD8明显升高,差异均有统计学意义(P<0.05)。结论草分枝杆菌F.U.36注射液肌肉注射对预防过敏性紫癜复发有一定的疗效,其机制可能与促进过敏性紫癜免疫异常恢复有关。

Objective To explore the effectiveness of Mycobacterium phlei injection in preventing the recurrence of Henoch - Schonlein purpura （HSP） and immunological function. Methods Totally 76 children with HSP were randomly divid- ed into Mycobacterium phlei therapy group （n = 38 ） and conventional therapy group （n = 38 ）. The two groups were all given routine treatment, whereas patients in the Mycobacterium phlei group were intramuscularly injected with Mycobacterium phlei in- jection （once a week for 12 weeks）. The recurrence rate in the two groups was observed six months and one year after treatment. The serum levels of IgA, IgG and IgM, the antigen markers of T - lymphocyte （ including CD3, CD4, CD8 and CD4/CD8 ） were detected before and after treatment. Another 15 healthy children were selected as normal control group. Data were analyzed using SPSS 13.0 software. Results The recurrence rate in Mycobacterium phlei therapy group was significantly lower than that in the conventional therapy group （P 〈0. 05）. The levels of IgA, CD3, CD4, and CD4/CDs in Mycobacterium phlei therapy group, conventional therapy group, and normal control group showed statistically significant differences before treatment （ P 〈 0. 05 ）. Compared with the normal control group, the level of IgA was significantly higher and the levels of CD3 , CD4 and CD4/CD8 were significantly lower in Mycobacterium phlei therapy group and conventional therapy group before treatment （ P 〈 0.05 ）. The levels of IgG, IgA, IgM, CD3, CD4, CD8, and CD4/CD8 between Mycobacterium phlei therapy group and conventional therapy group showed no significant differences before treatment （ P 〉 0.05 ）. After treatment, the level of IgA in Mycohacterium phlei therapy group was significantly lower than that in general therapy group （ P 〈 0.05 ） ; meanwhile, the levels of CD3, CD4 and CD4/CD8 in Mycobacterium phlei therapy group were significantly higher than those in conventional therapy group （ P 〈 0. 05 ）. Conclusion Mycobacterium Phlei injection is effective for the prevention of HSP recurrence, with a mechanism that may be re- lated with the promotion of the recovery of HSP immune abnormality.