几类小麦雄性不育系育性敏感期的可溶性蛋白质变化研究

Research on Soluble Protein Changes in Several Types of Wheat Male Sterile Line during the Fertility-sensitive Period

[目的]研究几种小麦雄性不育系育性敏感期的可溶性蛋白质含量变化。[方法]以2种K型小麦雄性不育系及保持系和YS型温敏不育系为材料,对花粉发育过程中倒二叶和花药中可溶性蛋白质含量进行研究。[结果]不育系花药中蛋白质含量从减数分裂期至单核期呈上升趋势,而单核期至三核期呈下降趋势,且单核期至二核期下降速率最快;保持系在单核期至三核期蛋白质含量下降较不育系缓慢,整个时期可溶性蛋白质含量明显低于保持系;不育系和保持系叶片中可溶性蛋白质含量在整个生育期变化没有花药中变化明显;K型不育系和YS型温敏不育系蛋白质含量差异不明显;不育系可溶性蛋白质含量显著下降。[结论]蛋白质合成受阻或较多的蛋白质降解会导致生理代谢紊乱,花粉正常发育受到阻碍。

[ Objective] To study the soluble protein changes in several types of wheat male sterile line during the fertility-sensitive period. [ Method ] Two K-types wheat male sterile lines, the maintainer line and the YS type temperature sensitive male sterile line were selected as the research objects, their soluble protein contents of the penultimate leaf and anther were studied in the process of pollen development. [ Result ] Protein content in the male sterile line anther tended to increase from meiosis to uninucleate stage, declined from uninucleate stage to trinucleate stage, and the descent rate was the fastest between uninucleate stage and binucleate stage among all stages. The protein content of maintainer line from uninucleate stage to trinucleate stage declined slower than that of the male sterile line, the soluble protein content of the male sterile line was significantly lower than that of the maintainer lines during the entire period. Soluble protein content of anther had more change than that of leaves of sterile line and maintainer line during the whole growth period. Protein content had no significant difference between the male sterile line and YS type temperature sensitive male sterile line. Soluble protein content of the male sterile line was found decreasing significantly. [ Conclusion] Protein synthesis blocked or more protein degradation will lead to physiological metabolism disorders, and the development of pollen will be hindered.