抗精子抗体检测的临床重要性

Clinical Importance of Screening Antisperm Antibodies in Semen

根据世界卫生组织的推荐,精子表面抗精子抗体(ASAB)的检测方法有混合抗球蛋白结合试验(MAR)、免疫珠试验(IBT)和精子-宫颈黏液接触试验(SCMC)等。这些方法不仅可以检测活动精子表面的抗体,而且能检测各种体液中的抗体,如血清、精浆和宫颈黏液等。输精管结扎后的男性是研究ASAB很好的模型。输精管结扎后男性的近睾端附睾液中的精子和输精管吻合后的精液中的精子都存在ASAB。通过回顾ASAB的发生与男性生殖道感染、精索静脉曲张和隐睾的关系,综述对男性不育患者进行ASAB检测的重要性。

According to the WHO guideline, tests for antisperm antibody （ASAB） on spermatozoa were recommended as： the mixed antiglobulin reaction（MAR）,the immunobead test （IBT） and sperm cervical mucus contact test （SCMC）. These tests can detect ASAB on mobile spermatozoa or in biological fluids such as serum, seminal plasma and cervical mucus and so on. Vasectomized men were the good model for the ASAB research. We used IBT and SCMC to detect ASAB on the surface of spermatozoa from the proximal vas deferens of vaseetomized men and the semen after vasovasostomy. The relationship between ASAB and infectious diseases of the male reproductive tract,varieocele and cryptorchidism were reviewed, so as to show the importance of screening semen ASAB in diagnosis of male infertility.