海金子中柽柳素-3-O-芸香糖苷和芦丁的分离及含量测定

Separation and determination of tamarix prime-3-O-rutinoside and rutin in Pittosporum. Illicioides Mak

目的研究海金子70%乙醇提取物的化学成分;建立HPLC测定海金子中柽柳素-3-O-芸香糖苷、芦丁的含量。方法采用硅胶柱色谱进行分离纯化,利用波谱技术、色谱技术进行结构鉴定。采用Agela Technologies PromosilC18（200 mm×4.6 mm,5μm）,流动相为甲醇-0.4%磷酸水溶液（34∶66）,流速为1.0 mL/min,检测波长为254 nm,柱温为40℃对不同采集时间、不同部位的海金子中的柽柳素-3-O-芸香糖苷、芦丁进行含量测定。结果分离鉴定了2个黄酮苷类化合物,分别为柽柳素-3-O-芸香糖苷和芦丁。柽柳素-3-O-芸香糖苷在0.254-1.145μg范围、芦丁在0.341-1.533μg范围与其峰面积呈良好的线性关系;平均回收率：柽柳素-3-O-芸香糖苷为100.08%,RSD为1.09%（n=6）,芦丁为99.89%,RSD为1.41%（n=6）。结论两种化合物首次从该植物中分离得到。所建立的HPLC简便、准确、重现性好,结果准确可靠,可用于海金沙中柽柳素-3-O-芸香糖苷和芦丁的测定。

Objective To investigate the chemical constituents in 70% alcohol extract of Pit- tosporum. Illicioides Mak. and develop an HPLC method for determination of tamarix prime-3-O- rutinoside and rutin in Pittosporum. Illicioides Mak. Methods The chemical constituents were iso- lated by column chromatography over silica dated by spectroscopic and chromatographi gel. The Structures of the two compounds were eluci- c analysis. Agela Technologies Promosil C18 column （200 mm×4.6 mm, 5 μm） was used with methanol-0.4% phosphoric acid （34：66） as mobile phase. The flow rate was 1.0 mL/min. The UV detection wavelength was 254 nm and the column temper- ature was 40 ℃. According to these conditions, the contents of tamarix prime-3-O-rutinoside and rutin in different parts of Pittosporum. Illicioides Mak cropped at different time were determined. Results The two flavone glycosides were identified as tamarix prime-3-O-rutinoside and rutin. The linear response range of tamarix prime-3-O-rutinoside and rutin were 0.254-1.145 μg and, 0.341 -1.533 μg, respectively. The average recoveries of these compounds were 100.08%（RSD= 1.09%） and 99.89%（RSD=1.41%）, respectively. Conclusion The two kinds of compounds were iso- lated from Pittosporum. Illicioides Mak for the first time. This established method in this study is simple, reliable, reproducible and accurate for the analysis of tamarix prime-3-O-rutinoside and rutin in Pittosporum. Illicioides Mak.