HPLC法测定痔浴净中苦参碱的含量

Determination of Matrine in Zhiyujing Mixtures by HPLC

目的建立痔浴净中苦参碱的含量测定方法。方法采用高效液相色谱法。SunFire C18色谱柱(4.6mm×150 mm,3.5μm),流动相为甲醇-水-三乙胺(48∶52∶0.05),检测波长为220 nm。结果此方法线性关系良好,平均回收率为100.29%。结论此方法简便、准确,可用于测定痔浴净中苦参碱的含量。

Purpose To establish a HPLC methed for the determination of Matrine in Zhiyujing Mix-tures. Methods The HPLC method was used at SunFire C18 column（4.6 mm× 150 mm,3.5μm）. The mobile phase consisted of methanol-water-triethylamine（48：52：0.05 ）. The detective wavelength was 220 nm. Results The linearity of this method was good with the average recovery of 100. 29%. Conclusion The method is simple and accurate. It can be used for the determination of the contents of Matrine in Zhiyujing Mixtures.