坏死性小肠结肠炎简易高效动物模型的建立

A simple and effective model for necrotizing enterocolitis

目的研究坏死性小肠结肠炎(NEC)动物模型的简易制作方法。方法新生大鼠称重后随机分为三组,分别予0.9%氯化钠注射液(NS)、150mmol/L乙酸(AA)、300mmol/LAA灌肠,观察其症状表现,24h后称重,然后断颈处死;取出近端结肠用以组织病理学评分,并留取血清标本做髓过氧化物酶(MPO)活性检测和白细胞介素6(IL-6)浓度检测。结果 300mmol/LAA组与NS组比较,MPO活性、IL-6浓度、组织病理学评分各方面明显增加,差异均有统计学意义(t分别=3.56、6.34,χ2=30.50,P均<0.05);试验前后24h体重增长明显减少,差异有统计学意义(t=3.75,P<0.05)。150mmol/LAA组与NS组比较,试验前后24h体重变化、MPO活性、IL-6浓度、组织病理学评分各方面,差异均无统计学意义(t分别=1.41、0.94、2.16,χ2=5.30,P均>0.05)。结论这种简单易行的方法能成功地建立NEC动物模型,且具有可重复性好、成功率高、建模周期短等特点,可应用于NEC的相关研究。

Objective To establish the simple and effective animal model of necrotizing enterocolitis. Methods The rats were weighed and randomized into three experimental groups, which were respectively given normal saline, acetic acid1 （150 retool/L） and acetic acid2 （300 mmol/L）. After 24 hours, all rats were weighed and then killed. The proximal colon was collected for histological examination. And blood serum was collected for measuring MPO activity and IL-6 concentration. Results The differences in weight change, MPO activity, IL-6 concentration and histological injure score between the 300 retool/L AA group and the NS group were statistically significant （t=3.75,3.56,6.34;X2=30.50,P〈0.05）. There had no statistic differences between the 150 retool/L group and the NS group in histological injure score, weight changes, MPO activity and IL-6 concentration （X2=5.30;t=l.41,0.94,2.16,P〉0.05）. Conclusions This method can establish the experimental animal model of necrotizing enterocolitis successfully, and it can be widely used in NEC related research because of repeatability, high success rate and short modeling cycle.