白内障摘除手术方式对兔眼房水中TNF-α、IL-1β、IL-6的影响

Investigate the effects of different operation ways of cataract affect levels of TNF-α, IL-1β和IL-6 in aqueoushumor

目的探讨不同白内障手术方式对兔眼房水中TNF-α、IL-1β和IL-6含量影响。方法 18只日本大耳白兔均分3组,I组空白对照组;II组超声乳化摘除术组;Ⅲ组超声乳化联合前段玻璃体切割术组。分别于术前,术后1、3、7、14、28 d对术眼进行临床检查,并抽取房水于-30℃中保存。用酶联免疫吸附试验对术后兔房水中的TNF-、IL-1β和IL-6含量进行测定。结果Ⅲ组后囊混浊程度较II组轻;兔房水TNF-α、IL-1β和IL-6含量于术后3、7、14 d和28 d明显升高,术后7～14 d达最高值;Ⅲ组房水中TNF-α、IL-1β和IL-6含量较II组的稍低,具有统计学意义。结论超声乳化联合前段玻璃体切割术治疗后发性白内障的效果优于单纯的超声乳化摘除术,在一定程度阻止后发障的发生。超声乳化联合前段玻璃体切割术后的TNF-α、IL-1β和IL-6的含量较单纯的超声乳化摘除术的低,TNF-α、IL-1β和IL-6可能参与后发性白内障的形成。

Ojbective Investigate the effects of different opemtion ways of cataract affect levels of TNF - α , IL-1β and IL-6 in aqueous humor. Methods Eighteen rabbits were divided into three groups, Group I was control ; Group Ⅱ was operated by phacoemtdsification; Group Ⅲ was phacoemulsification combined with anterior vitrectomy. In postoperativel, 3,7,14 and 28day,all operational rabbit eyes were observed via microscope, aqueous humor was drawn and eonservated in - 30 ℃ for determining TNF-α, IL-1β and IL-6 content by enzyme-linked immunosorbent assay. Results Extent of poste- rior capsular opacification is ligher in Group Ⅲ than in Group II. Lever of I TNF-α,IL-1β and L-6 in aqueous humor is obviously increased in posteroperated for 3 ,7,14 and 28day. Lever of I TNF-α,IL-1β and L-6 in aqueoxm humor for Group Ⅲ is lower than Group II, which has statistics significant. Conclusion Phacoemulsification combined with anterior vitrectomy cure posterior capsular opacification which is superior to phacoemu]s/fication, prevent happening posterior capsular opacifi- cation to some extent. Postoperative lever of TNF-α, IL-1β and IL-6 for phacoemulsification combined with anterior vitrecto- my is lower than phacoemulsification, TNF-α,IL-1β and IL-6 may be take part in formation of posterior capsular opacification.