术后肠梗阻大鼠模型的建立

The establishment of postoperative intestinal obstruction model in rats

目的探索建立一种大鼠的术后肠梗阻模型的方法。方法选取健康成年Wistar大鼠60只,随机分为正常对照组25只,模型组35只。对照组正常进食水,模型组大鼠剖腹后,取出全小肠,用盐水棉球自下而上反复多次擦拭肠管,持续时间约10 min之后将全部肠管还纳入腹,关腹。手术24 h后伊文氏蓝染色剂推进法检测肠道传输功能。结果模型组大鼠死亡6只,与对照组相比,模型组大鼠的肠道传输速率明显减慢(P<0.05)。结论此种方法能够成功建立术后肠梗阻大鼠模型,方法简单可靠,建模时间较短,可应用于较多临床及基础实验中。

Objective To establish a rat model of postoperative ileus. Methods Totally 60 healthy adult Wistar rats were randomly divided into the control group （n = 25 ） and the model group （n = 35 ）. The rats in the control group were given a normal diet while those in the model group were underwent laparotomy to take the small intestine out of the abdominal cavity. The small intestine which was carefully wiped saline cotton from the cecum to the duodenal six times repeatedly within 10 minutes were returned to the abdominal cavity and the surgical wound was sutured. The intestinal transit was measured by Evans blue dye migration at 24 hours after the operation. Results Six rats with died in the model group. The intestinal transit in the model group was obviously slower compared with that in the control group （ P 〈 0.05 ）. Conclusion The rat model of postoperative ileus can be successfully established through this method, which is simple, reliable and can be used in many clinical and basic experiments.