耐亚安培南的铜绿假单胞菌OprD2的缺失与金属β-内酰胺酶编码基因的研究

Investigation of the deficiency of OprD2 in Imipenem resistant Pseudomonas aeruginosa and the genotype of encoding gene of Metal beta-Lactamases

目的了解临床分离的耐亚胺培南铜绿假单胞菌中外膜蛋白OprD2的缺失和金属β-内酰胺酶(MBLs)的基因型,为临床治疗和医院感染提供依据。方法用亚胺培南—EDTA纸片增效法筛选出产MBLs的菌株,并用PCR检测MBLs的基因和OprD2基因。结果 76株耐亚胺培南的铜绿假单胞菌中有16株产MBLs(检出率21.1%),其中VIM-2型13株(检出率17.1%),IMP-1型3株(检出率3.9%),孔蛋白OprD2缺失51株﹙检出率75%﹚。结论临床分离的耐亚胺培南的铜绿假单胞菌大多存在外膜蛋白OprD2的缺失,产生VIM-2型和IMP-1型MBLs菌株在本院有一定的流行,应密切监测其耐药变化。

Objective To explore the deficiency of outer membrane protein OprD2 in Imipenem resistant Pseudomonas aeruginosa and the genotype of encoding gene of Metal beta-lactamases from clinically isolated strains,and to provide the basis for clinical therapy and hospital infection.Methods MBLs producing strains were screened with Imipenem-EDTA paper synergistic method.MBLs and OprD2 were detected by PCR.Results 16 among the 76 strains of Imipenem resistant Pseudomonas aeruginosa produced MBLs（with the detection rate of 21.1%）,with VIM-2 type 13 strains（with the detection rate of 17.1%）,IMP-1 type 3 strains（with the detection rate of 3.9%）,Porin OprD2O deficiency 51 strains﹙with the detection rate of 75%﹚.Conclusion The deficiency of outer membrane protein OprD2 existed in most of the clinically isolated Imipenem resistant Pseudomonas aeruginosa,with some popularity of VIM-2 and VIM-2 producing MBLs strains,the change of resistance of the strains should be monitored closely.