IL-6、IL-6R和IL-4多态性与上海地区汉族人群慢性牙周炎患者的关联研究

Analysis of correlation between IL-6,IL-6R and IL-4 single nucleotide polymorphism and the susceptibility of chronic periodontitis among Shanghai patients of Han nationality

目的研究IL-6、IL-6R和IL-4基因组单核苷酸多态性(single nucleotide polymorphisms,SNPs)与上海地区汉族人群慢性牙周炎患者的相关性。方法应用TaqMan荧光实时定量PCR方法,对128例牙周正常者和198例中度以上慢性牙周炎患者的IL-6-572G/C、IL-6R 48892A/C和IL-4-590C/T等3个单核苷酸多态性位点进行分析。应用SPSS 17.0软件包对数据进行χ2检验分析。结果分析显示这3个位点的基因型频率与等位基因频率在牙周正常组和慢性牙周炎组间的分布差异均无显著性。结论 IL-6-572G/C、IL-6R 48892A/C和IL-4-590C/T基因组单核苷酸多态性位点与上海地区汉族人群的慢性牙周炎易感性无显著相关性。

Objective To investigate the correlation between IL-6,IL-6R and IL-4 single nucleotide polymorphisms （SNPs） and the susceptibility of chronic periodontitis among Shanghai patients of Han nationality. Methods Through the application of TaqMan probe real-time fluorescent quantitative PCR, 128 health control subjects and 198 patients with chronic periodontitis（CP） above moderate degree were investigated from three single nucleotide polymorphisms sites, namely IL-6 -572G/C, IL-6R 48892A/C and IL-d -590C/T. Statistical analysis of Chi square test was performed with SPSS 17.0 software package. Results In the screening of the 3 target SNPs, there were no statistically significant differences in distribution of the genotypes and alleles between healthy controls and CP patients. Conclusions The result suggests that the SNPs gene locus of IL-6 -572G/C,IL-6R 48892A/C and IL-4 -590C/T may not be signifi- cantly associated with the susceptibility of chronic periodontitis among Shanghai patients of I-fan nationality.