肺炎衣原体(Cpn)诱发肺癌大鼠模型

Preliminary study on lung cancer rat model produced by Chlamydia pneumoniae(Cpn)

目的初步探索肺炎衣原体(Chlamydia pneumoniae,Cpn)诱发大鼠肺癌模型的建立。方法应用多次气管内注入Cpn菌液(TW-183)的方法试制肺癌大鼠模型,微量免疫荧光(microimmunofluorescence,MIF)法检测大鼠血清中Cpn特异性抗体,PCR检测肺癌组织中Cpn-DNA,酶联免疫法检测肺癌组织中Cpn特异性抗原。结果单独Cpn感染组Wistar鼠的Cpn感染率为72.9%(35/48),Cpn感染联合苯并芘(benzopyrene,Bp)组的Cpn感染率为76.7%(33/43),两组的肺癌发生率分别为25.0%(12/48)和44.2%(19/43),与正常对照组相比,P值分别为0.001和0.000。结论 Cpn感染诱发大鼠肺癌模型的建立是成功的,有助于肺癌的防治及其发病机制研究。

Objective Preliminary study on rat model of lung cancer produced by Chlamydia pneumoniae （Cpn）. Methods The lung cancer animal model was developed by intratracheal repeated injecting Cpn （TW-183） into lung of rat. The Cpn-antibodies （Cpn-IgA, -IgG and -IgM） of rat serum were measured by microimmunofluorescence （MIF） method. The Cpn-DNA or Cpn-Ag of rat lung cancer were detected by PCR method or ELISA. Results The prevalence of Cpn infection was 72. 9~ （35/48） in Cpn group, and 76.7~ （33/43） in Cpn plus benzopyrene （Bp） group. The incidences of lung carcinomas of the two groups were 25.0~ （12/48） and 44.2% （19/43）. Compared with normal controls,P value were 0. 001 and 0. 000, respectively. Conclusions A rat model of lung carcinoma produced by Cpn was successfully prepared for further study in experiment on treatment, prevention and mechanism.